Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13861 and 13862 are separated by 244 nucleotides13862 and 13863 are separated by 270 nucleotides13863 and 13864 are separated by 455 nucleotides13864 and 13865 are separated by 2433 nucleotides mRNA_5493: 13861 - KOG0297 TNF receptor-associated factor, at 226,930 to 227,786 13861 mRNA_5494: 13862 - KOG1119 Mitochondrial Fe-S cluster biosynthesis protein ISA2 (contains a HesB-like domain), at 228,031 to 229,041 13862 mRNA_5495: 13863 - K10733 GINS2, PSF2 GINS complex subunit 2, at 229,312 to 230,460 13863 mRNA_5496: 13864 - HMMPfam-HSF-type DNA-binding-PF00447,SUPERFAMILY--SSF46785, at 230,916 to 233,624 13864 mRNA_5497: 13865 - KOG3872 FOG FHA domain, at 236,058 to 237,247 13865
Group Condition mRNA_5493 mRNA_5494 mRNA_5495 mRNA_5496 mRNA_5497
13861 13862 13863 13864 13865
auxotrophy YNB Glucose +0.0 N.D. N.D. -0.6 -0.5
facs on lipid accumulation Sorted High BODIPY Gate -0.7 N.D. N.D. -0.3 +0.2
growth on ypd YPD -0.5 N.D. N.D. -0.7 +0.3
growth on ricinoleic acid YNB Glucose -0.1 N.D. N.D. -0.1 -0.6
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. N.D. -0.2 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 N.D. N.D. -0.7 +0.2
auxotrophy Dropout Complete -0.1 N.D. N.D. +0.3 -0.5
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. N.D. +0.2 -0.5
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. N.D. +0.0 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 N.D. N.D. -0.0 +0.4
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. N.D. +0.5 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 N.D. N.D. +0.6 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 N.D. N.D. +0.4 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. N.D. +0.7 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. N.D. +0.0 +0.6
facs on lipid accumulation Sorted Low BODIPY Gate -0.7 N.D. N.D. +1.0 +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. N.D. +0.8 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 N.D. N.D. +0.9 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 N.D. N.D. +1.1 +0.0
growth on oleic acid YNB Oleic Acid +0.0 N.D. N.D. +1.3 -0.2
growth on oleic acid YNB Glucose +0.1 N.D. N.D. +1.4 +0.1
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