Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13863 and 13864 are separated by 455 nucleotides13864 and 13865 are separated by 2433 nucleotides13865 and 13866 are separated by 1518 nucleotides13866 and 13867 are separated by 379 nucleotides mRNA_5495: 13863 - K10733 GINS2, PSF2 GINS complex subunit 2, at 229,312 to 230,460 13863 mRNA_5496: 13864 - HMMPfam-HSF-type DNA-binding-PF00447,SUPERFAMILY--SSF46785, at 230,916 to 233,624 13864 mRNA_5497: 13865 - KOG3872 FOG FHA domain, at 236,058 to 237,247 13865 mRNA_5498: 13866 - Hypothetical Protein, at 238,766 to 240,187 13866 mRNA_5499: 13867 - KOG1208 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 240,567 to 242,106 13867
Group Condition mRNA_5495 mRNA_5496 mRNA_5497 mRNA_5498 mRNA_5499
13863 13864 13865 13866 13867
auxotrophy YNB Glucose N.D. -0.6 -0.5 -0.2 -0.5
growth on ricinoleic acid YNB Glucose N.D. -0.1 -0.6 -0.2 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.7 +0.2 +0.1 -0.5
auxotrophy YNB Glucose 75mM Methionine N.D. +0.0 -0.4 -0.1 -0.6
auxotrophy Dropout Complete N.D. +0.3 -0.5 -0.3 -0.5
auxotrophy YNB Glucose 75mM Arginine N.D. -0.2 -0.4 -0.1 -0.1
growth on ypd YPD N.D. -0.7 +0.3 +0.1 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.7 -0.2 -0.3 -0.7
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.9 -0.1 -0.4 -0.6
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.2 -0.5 -0.1 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.3 +0.2 +0.2 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.5 -0.5 -0.0 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.8 -0.3 -0.0 +0.0
growth on oleic acid YNB Oleic Acid N.D. +1.3 -0.2 +0.0 -0.2
growth on oleic acid YNB Glucose N.D. +1.4 +0.1 -0.1 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.6 -0.2 +0.1 +0.5
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.0 +0.6 +0.2 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.0 +0.4 -0.1 +0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.4 +0.3 -0.0 +0.6
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +1.0 +0.3 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +1.1 +0.0 +0.2 +0.7
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