Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13871 and 13872 are separated by 454 nucleotides13872 and 13873 are separated by 355 nucleotides13873 and 13874 are separated by 1562 nucleotides13874 and 13875 are separated by 98 nucleotides mRNA_5503: 13871 - HMMPfam-Chitin synthase III catalytic subunit-PF12271, at 251,563 to 253,057 13871 mRNA_5504: 13872 - KOG4034 Uncharacterized conserved protein NOF (Neighbor of FAU), at 253,512 to 254,101 13872 mRNA_5505: 13873 - KOG0297 TNF receptor-associated factor, at 254,457 to 255,755 13873 mRNA_5506: 13874 - KOG2504 Monocarboxylate transporter, at 257,318 to 259,586 13874 mRNA_5507: 13875 - KOG0134 NADH-flavin oxidoreductase/12-oxophytodienoate reductase, at 259,685 to 261,151 13875
Group Condition mRNA_5503 mRNA_5504 mRNA_5505 mRNA_5506 mRNA_5507
13871 13872 13873 13874 13875
ypd buoyancy 40 hrs Median Buoyancy -1.0 N.D. -0.0 -0.1 -0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.8 N.D. +0.2 +0.0 -0.7
ypd buoyancy 40 hrs Low Buoyancy -0.4 N.D. -0.4 +0.1 -0.4
ypd buoyancy 40 hrs High Buoyancy -0.8 N.D. +0.1 +0.1 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 N.D. -0.1 +0.0 -1.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 N.D. -0.4 +0.5 -1.0
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. -0.3 -0.1 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 N.D. +0.1 -0.5 +0.8
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 N.D. -0.8 +0.3 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.5 N.D. -0.0 -0.1 -0.4
auxotrophy Dropout Complete +0.5 N.D. +0.1 -0.0 -0.4
growth on ricinoleic acid YNB Glucose +0.5 N.D. +0.0 +0.1 -0.4
growth on oleic acid YNB Glucose +0.5 N.D. -0.1 +0.3 -0.4
growth on oleic acid YNB Oleic Acid +0.7 N.D. -0.1 +0.3 -0.5
auxotrophy YNB Glucose 75mM Arginine +0.7 N.D. -0.2 +0.2 -0.1
auxotrophy YNB Glucose +0.9 N.D. -0.1 +0.0 +0.3
growth on ypd YPD +0.1 N.D. -0.0 +0.3 +1.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. -0.1 -0.3 +2.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 N.D. +0.0 +0.1 +1.9
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.6 N.D. +0.3 +0.1 +2.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. -0.2 +0.4 +2.2
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