Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13879 and 13880 are separated by 456 nucleotides13880 and 13881 are separated by 531 nucleotides13881 and 13882 are separated by 122 nucleotides13882 and 13883 are separated by 377 nucleotides mRNA_5511: 13879 - ProSiteProfiles-Zinc finger RING-type profile.-PS50089,ProSiteProfiles-Zinc finger TRAF-type profile.-PS50145,SUPERFAMILY--SSF49599,SUPERFAMILY--SSF57850, at 267,375 to 268,513 13879 mRNA_5512: 13880 - HMMPfam-PB1 domain-PF00564,HMMPfam-CBS domain-PF00571,ProSiteProfiles-CBS domain profile.-PS51371,SMART-Domain in cystathionine beta-synthase and other proteins.-SM00116,SUPERFAMILY--SSF54277,SUPERFAMILY--SSF54631, at 268,970 to 271,571 13880 mRNA_5513: 13881 - KOG4178 Soluble epoxide hydrolase, at 272,103 to 273,154 13881 mRNA_5514: 13882 - K03031 PSMD8, RPN12 26S proteasome regulatory subunit N12, at 273,277 to 274,802 13882 mRNA_5515: 13883 - K02738 PSMB6 20S proteasome subunit beta 1, at 275,180 to 276,108 13883
Group Condition mRNA_5511 mRNA_5512 mRNA_5513 mRNA_5514 mRNA_5515
13879 13880 13881 13882 13883
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 N.D. -0.1 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.0 N.D. -0.5 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. -0.4 N.D. N.D.
growth on oleic acid YNB Glucose -0.3 N.D. -0.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 N.D. +0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. -0.1 N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.2 N.D. -0.0 N.D. N.D.
auxotrophy YNB Glucose -0.2 N.D. -0.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 N.D. +0.3 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. -0.0 N.D. N.D.
auxotrophy Dropout Complete -0.3 N.D. +0.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. +0.1 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. +0.1 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. +0.2 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. -0.0 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. +0.2 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 N.D. +0.3 N.D. N.D.
growth on ricinoleic acid YNB Glucose -0.2 N.D. +0.4 N.D. N.D.
growth on ypd YPD +0.2 N.D. +0.2 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. +0.3 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. +0.4 N.D. N.D.
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