Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13880 and 13881 are separated by 531 nucleotides13881 and 13882 are separated by 122 nucleotides13882 and 13883 are separated by 377 nucleotides13883 and 13884 are separated by 155 nucleotides mRNA_5512: 13880 - HMMPfam-PB1 domain-PF00564,HMMPfam-CBS domain-PF00571,ProSiteProfiles-CBS domain profile.-PS51371,SMART-Domain in cystathionine beta-synthase and other proteins.-SM00116,SUPERFAMILY--SSF54277,SUPERFAMILY--SSF54631, at 268,970 to 271,571 13880 mRNA_5513: 13881 - KOG4178 Soluble epoxide hydrolase, at 272,103 to 273,154 13881 mRNA_5514: 13882 - K03031 PSMD8, RPN12 26S proteasome regulatory subunit N12, at 273,277 to 274,802 13882 mRNA_5515: 13883 - K02738 PSMB6 20S proteasome subunit beta 1, at 275,180 to 276,108 13883 mRNA_5516: 13884 - Hypothetical Protein, at 276,264 to 277,474 13884
Group Condition mRNA_5512 mRNA_5513 mRNA_5514 mRNA_5515 mRNA_5516
13880 13881 13882 13883 13884
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 N.D. N.D. -0.9
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.2 N.D. N.D. -0.7
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 N.D. N.D. -0.7
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.5 N.D. N.D. +0.2
auxotrophy Dropout Complete N.D. +0.2 N.D. N.D. -0.4
growth on oleic acid YNB Oleic Acid N.D. -0.0 N.D. N.D. -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 N.D. N.D. -0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.4 N.D. N.D. +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 N.D. N.D. -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 N.D. N.D. -0.0
growth on oleic acid YNB Glucose N.D. -0.0 N.D. N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 N.D. N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.3 N.D. N.D. -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 N.D. N.D. +0.1
growth on ricinoleic acid YNB Glucose N.D. +0.4 N.D. N.D. -0.2
growth on ypd YPD N.D. +0.2 N.D. N.D. +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 N.D. N.D. +0.2
auxotrophy YNB Glucose N.D. -0.0 N.D. N.D. +0.3
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 N.D. N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.3 N.D. N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.4 N.D. N.D. +0.7
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