Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13893 and 13894 are separated by 239 nucleotides13894 and 13895 are separated by 420 nucleotides13895 and 13896 are separated by 279 nucleotides13896 and 13897 are separated by 244 nucleotides mRNA_5525: 13893 - HMMPfam-Xyloglucan fucosyltransferase-PF03254, at 299,170 to 301,469 13893 mRNA_5526: 13894 - K00052 leuB 3-isopropylmalate dehydrogenase, at 301,709 to 303,276 13894 mRNA_5527: 13895 - K11883 NOB1 RNA-binding protein NOB1, at 303,697 to 305,493 13895 mRNA_5528: 13896 - HMMPfam-Protein of unknown function (DUF2416)-PF10315, at 305,773 to 306,425 13896 mRNA_5529: 13897 - K18045 SIW14, OCA3 tyrosine-protein phosphatase SIW14, at 306,670 to 307,631 13897
Group Condition mRNA_5525 mRNA_5526 mRNA_5527 mRNA_5528 mRNA_5529
13893 13894 13895 13896 13897
growth on oleic acid YNB Oleic Acid +0.0 N.D. N.D. -0.6 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. N.D. -0.2 -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. N.D. -0.1 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 N.D. N.D. -0.6 -0.1
growth on oleic acid YNB Glucose -0.1 N.D. N.D. -0.0 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. N.D. -0.5 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 N.D. N.D. -0.4 +0.3
auxotrophy Dropout Complete +0.0 N.D. N.D. -0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. N.D. -0.3 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. N.D. -0.0 +0.3
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. N.D. -0.0 +0.3
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. N.D. +0.0 +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.0 N.D. N.D. -0.3 +0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. N.D. +0.1 +0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.0 N.D. N.D. +0.1 +0.3
auxotrophy YNB Glucose -0.0 N.D. N.D. -0.0 +0.4
growth on ypd YPD +0.0 N.D. N.D. +0.1 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. N.D. +0.2 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. N.D. +0.4 -0.2
growth on ricinoleic acid YNB Glucose +0.1 N.D. N.D. +0.1 +0.7
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 N.D. N.D. +0.6 +0.3
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