Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13897 and 13898 are separated by 2026 nucleotides13898 and 13899 are separated by 635 nucleotides13899 and 13900 are separated by 36 nucleotides13900 and 13901 are separated by 172 nucleotides mRNA_5529: 13897 - K18045 SIW14, OCA3 tyrosine-protein phosphatase SIW14, at 306,670 to 307,631 13897 mRNA_5530: 13898 - K02949 RP-S11e, RPS11 small subunit ribosomal protein S11e, at 309,658 to 310,320 13898 mRNA_5531: 13899 - K03800 lplA, lplJ lipoate---protein ligase, at 310,956 to 312,571 13899 mRNA_5532: 13900 - K01519 ITPA inosine triphosphate pyrophosphatase, at 312,608 to 313,852 13900 mRNA_5533: 13901 - Hypothetical Protein, at 314,025 to 315,738 13901
Group Condition mRNA_5529 mRNA_5530 mRNA_5531 mRNA_5532 mRNA_5533
13897 13898 13899 13900 13901
ypd buoyancy 40 hrs Low Buoyancy -0.6 N.D. N.D. -0.4 -1.2
growth on oleic acid YNB Glucose -0.2 N.D. N.D. -0.3 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 N.D. N.D. -0.3 -0.8
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. N.D. -0.1 -0.6
growth on oleic acid YNB Oleic Acid -0.2 N.D. N.D. +0.0 -0.5
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. N.D. +0.0 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. N.D. +0.0 -0.5
auxotrophy Dropout Complete +0.1 N.D. N.D. -0.2 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 N.D. N.D. -0.1 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. N.D. +0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. N.D. -0.4 +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. N.D. -0.3 +0.4
growth on ypd YPD +0.2 N.D. N.D. +0.6 -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. N.D. +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 N.D. N.D. +0.1 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. N.D. +0.2 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 N.D. N.D. +0.2 +0.3
auxotrophy YNB Glucose +0.4 N.D. N.D. +0.1 +0.3
growth on ricinoleic acid YNB Glucose +0.7 N.D. N.D. +0.4 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 N.D. N.D. +0.0 +0.7
facs on lipid accumulation Sorted High BODIPY Gate +0.6 N.D. N.D. -0.1 +0.9
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