Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13898 and 13899 are separated by 635 nucleotides13899 and 13900 are separated by 36 nucleotides13900 and 13901 are separated by 172 nucleotides13901 and 13902 are separated by 197 nucleotides mRNA_5530: 13898 - K02949 RP-S11e, RPS11 small subunit ribosomal protein S11e, at 309,658 to 310,320 13898 mRNA_5531: 13899 - K03800 lplA, lplJ lipoate---protein ligase, at 310,956 to 312,571 13899 mRNA_5532: 13900 - K01519 ITPA inosine triphosphate pyrophosphatase, at 312,608 to 313,852 13900 mRNA_5533: 13901 - Hypothetical Protein, at 314,025 to 315,738 13901 mRNA_5534: 13902 - K18798 AFG1, LACE1 peroxisome-assembly ATPase, at 315,936 to 317,983 13902
Group Condition mRNA_5530 mRNA_5531 mRNA_5532 mRNA_5533 mRNA_5534
13898 13899 13900 13901 13902
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.4 -1.2 -0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.0 -0.5 -1.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.1 -0.6 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.3 -0.8 +0.2
growth on oleic acid YNB Glucose N.D. N.D. -0.3 -0.4 -0.1
growth on ypd YPD N.D. N.D. +0.6 -0.5 -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.1 +0.1 -0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.0 -0.5 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.3 +0.4 -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.0 -0.2 +0.2
auxotrophy Dropout Complete N.D. N.D. -0.2 -0.2 +0.6
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.1 +0.9 -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.1 +0.4 -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.2 +0.3 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.1 -0.1 +0.4
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.4 -0.2 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.1 -0.0 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.2 +0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.4 +0.5 +0.6
auxotrophy YNB Glucose N.D. N.D. +0.1 +0.3 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.0 +0.7 +0.7
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