Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13911 and 13912 are separated by 640 nucleotides13912 and 13913 are separated by 1199 nucleotides13913 and 13914 are separated by 134 nucleotides13914 and 13915 are separated by 266 nucleotides mRNA_5543: 13911 - K15190 MEPCE, BCDIN3 7SK snRNA methylphosphate capping enzyme, at 338,637 to 339,590 13911 mRNA_5544: 13912 - Hypothetical Protein, at 340,231 to 341,133 13912 mRNA_5545: 13913 - Hypothetical Protein, at 342,333 to 343,433 13913 mRNA_5546: 13914 - Hypothetical Protein, at 343,568 to 344,161 13914 mRNA_5547: 13915 - K08994 yneE putative membrane protein, at 344,428 to 346,193 13915
Group Condition mRNA_5543 mRNA_5544 mRNA_5545 mRNA_5546 mRNA_5547
13911 13912 13913 13914 13915
facs on lipid accumulation Sorted High BODIPY Gate -0.8 -0.0 -1.2 -0.4 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +1.1 -2.3 -0.4 +0.5 -1.0
facs on lipid accumulation Sorted No BODIPY Gate -0.7 -0.0 -0.3 -0.4 -0.0
growth on oleic acid YNB Glucose -0.0 -0.1 -0.3 -0.2 -0.4
growth on oleic acid YNB Oleic Acid +0.1 -0.3 -0.2 -0.3 -0.2
auxotrophy Dropout Complete -0.7 +0.3 +0.4 -0.5 -0.3
growth on ricinoleic acid YNB Glucose +0.2 -0.5 +0.2 -0.1 -0.5
growth on ypd YPD -0.9 -0.2 +0.6 -0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.7 -0.6 -0.0 -0.1 -0.4
auxotrophy YNB Glucose -0.4 +0.2 +0.4 -0.3 -0.3
auxotrophy YNB Glucose 75mM Arginine +0.4 +0.1 -0.2 -0.2 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.6 -0.1 -0.2 +0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.8 -0.8 +0.2 +0.0 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.4 +0.2 +0.0 -0.3 -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.3 -0.4 +0.1 +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.2 -0.1 +0.3 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.8 -0.5 +0.4 +0.1 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.4 +0.0 +0.2 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.6 -0.3 +0.3 -0.2 -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.5 -0.1 -0.2 -0.6 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +1.5 -0.2 +0.4 -0.2 -0.3
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