Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13918 and 13919 are separated by 343 nucleotides13919 and 13920 are separated by 872 nucleotides13920 and 13921 are separated by 201 nucleotides13921 and 13922 are separated by 602 nucleotides mRNA_5550: 13918 - K09424 REB1 Myb-like DNA-binding protein REB1, at 353,365 to 355,997 13918 mRNA_5551: 13919 - ProSitePatterns-Tyrosine protein kinases specific active-site signature.-PS00109,SUPERFAMILY--SSF56112, at 356,341 to 358,990 13919 mRNA_5552: 13920 - K03360 GRR1 F-box and leucine-rich repeat protein GRR1, at 359,863 to 362,993 13920 mRNA_5553: 13921 - K20791 NAA10_11, ARD1_2 N-alpha-acetyltransferase 10/11, at 363,195 to 364,083 13921 mRNA_5554: 13922 - KOG0979 Structural maintenance of chromosome protein SMC5/Spr18, SMC superfamily, at 364,686 to 369,253 13922
Group Condition mRNA_5550 mRNA_5551 mRNA_5552 mRNA_5553 mRNA_5554
13918 13919 13920 13921 13922
facs on lipid accumulation Sorted High BODIPY Gate -2.0 -0.4 -0.9 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -1.3 -0.5 -1.4 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -1.8 +0.0 -0.1 N.D. N.D.
growth on ypd YPD -2.5 -0.2 +0.8 N.D. N.D.
auxotrophy Dropout Complete -0.2 +0.2 -1.6 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy -1.0 -0.1 -0.3 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.8 -0.0 +0.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.2 -0.8 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.9 +0.2 -1.7 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.6 +0.0 +0.1 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -0.3 +0.4 -0.5 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.5 +0.1 -0.9 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.4 -0.8 N.D. N.D.
growth on oleic acid YNB Glucose +0.4 -0.0 -0.4 N.D. N.D.
growth on ricinoleic acid YNB Glucose -0.0 +0.0 +0.1 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -1.1 -0.1 +1.3 N.D. N.D.
auxotrophy YNB Glucose +0.9 +0.2 -0.9 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.8 -0.2 -0.3 N.D. N.D.
growth on oleic acid YNB Oleic Acid +0.9 +0.1 -0.5 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +1.3 +0.3 -0.7 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 -0.3 +1.9 N.D. N.D.
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