Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13919 and 13920 are separated by 872 nucleotides13920 and 13921 are separated by 201 nucleotides13921 and 13922 are separated by 602 nucleotides13922 and 13923 are separated by 199 nucleotides mRNA_5551: 13919 - ProSitePatterns-Tyrosine protein kinases specific active-site signature.-PS00109,SUPERFAMILY--SSF56112, at 356,341 to 358,990 13919 mRNA_5552: 13920 - K03360 GRR1 F-box and leucine-rich repeat protein GRR1, at 359,863 to 362,993 13920 mRNA_5553: 13921 - K20791 NAA10_11, ARD1_2 N-alpha-acetyltransferase 10/11, at 363,195 to 364,083 13921 mRNA_5554: 13922 - KOG0979 Structural maintenance of chromosome protein SMC5/Spr18, SMC superfamily, at 364,686 to 369,253 13922 mRNA_5555: 13923 - K19984 EXOC5, SEC10 exocyst complex component 5, at 369,453 to 373,843 13923
Group Condition mRNA_5551 mRNA_5552 mRNA_5553 mRNA_5554 mRNA_5555
13919 13920 13921 13922 13923
auxotrophy Dropout Complete +0.2 -1.6 N.D. N.D. -0.6
auxotrophy YNB Glucose +0.2 -0.9 N.D. N.D. -0.5
facs on lipid accumulation Sorted High BODIPY Gate -0.4 -0.9 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.8 N.D. N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.8 N.D. N.D. -0.4
auxotrophy YNB Glucose 75mM Methionine +0.4 -0.5 N.D. N.D. -0.7
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 -1.4 N.D. N.D. +1.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 -0.1 N.D. N.D. -0.6
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.3 N.D. N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.9 N.D. N.D. +0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -1.7 N.D. N.D. +1.2
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.3 N.D. N.D. +0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.0 +0.0 N.D. N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 +1.3 N.D. N.D. -1.2
growth on oleic acid YNB Glucose -0.0 -0.4 N.D. N.D. +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 +0.1 N.D. N.D. +0.3
growth on oleic acid YNB Oleic Acid +0.1 -0.5 N.D. N.D. +0.9
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 -0.7 N.D. N.D. +1.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +1.9 N.D. N.D. -0.8
growth on ricinoleic acid YNB Glucose +0.0 +0.1 N.D. N.D. +0.9
growth on ypd YPD -0.2 +0.8 N.D. N.D. +0.6
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