Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13921 and 13922 are separated by 602 nucleotides13922 and 13923 are separated by 199 nucleotides13923 and 13924 are separated by 387 nucleotides13924 and 13925 are separated by 323 nucleotides mRNA_5553: 13921 - K20791 NAA10_11, ARD1_2 N-alpha-acetyltransferase 10/11, at 363,195 to 364,083 13921 mRNA_5554: 13922 - KOG0979 Structural maintenance of chromosome protein SMC5/Spr18, SMC superfamily, at 364,686 to 369,253 13922 mRNA_5555: 13923 - K19984 EXOC5, SEC10 exocyst complex component 5, at 369,453 to 373,843 13923 mRNA_5556: 13924 - KOG4580 Component of vacuolar transporter chaperone (Vtc) involved in vacuole fusion, at 374,231 to 375,171 13924 mRNA_5557: 13925 - K09008 NDUFAF3 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3, at 375,495 to 376,430 13925
Group Condition mRNA_5553 mRNA_5554 mRNA_5555 mRNA_5556 mRNA_5557
13921 13922 13923 13924 13925
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.3 N.D. -1.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.1 N.D. -0.9
auxotrophy YNB Glucose N.D. N.D. -0.5 N.D. -0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +1.2 N.D. -2.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.6 N.D. -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.7 N.D. -0.1
auxotrophy Dropout Complete N.D. N.D. -0.6 N.D. -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +1.3 N.D. -2.0
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.4 N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.2 N.D. -0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -1.2 N.D. +0.7
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.4 N.D. -0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.8 N.D. +0.7
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 N.D. +0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.9 N.D. -0.8
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.9 N.D. -0.8
growth on oleic acid YNB Glucose N.D. N.D. +0.5 N.D. -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.3 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.0 N.D. +0.2
growth on ypd YPD N.D. N.D. +0.6 N.D. +0.5
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +1.1 N.D. +0.2
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