Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13922 and 13923 are separated by 199 nucleotides13923 and 13924 are separated by 387 nucleotides13924 and 13925 are separated by 323 nucleotides13925 and 13926 are separated by 435 nucleotides mRNA_5554: 13922 - KOG0979 Structural maintenance of chromosome protein SMC5/Spr18, SMC superfamily, at 364,686 to 369,253 13922 mRNA_5555: 13923 - K19984 EXOC5, SEC10 exocyst complex component 5, at 369,453 to 373,843 13923 mRNA_5556: 13924 - KOG4580 Component of vacuolar transporter chaperone (Vtc) involved in vacuole fusion, at 374,231 to 375,171 13924 mRNA_5557: 13925 - K09008 NDUFAF3 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3, at 375,495 to 376,430 13925 mRNA_5558: 13926 - KOG3328 HGG motif-containing thioesterase, at 376,866 to 377,685 13926
Group Condition mRNA_5554 mRNA_5555 mRNA_5556 mRNA_5557 mRNA_5558
13922 13923 13924 13925 13926
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.3 N.D. -1.0 -0.5
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 N.D. -0.9 -0.9
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -1.2 N.D. +0.7 -0.8
auxotrophy YNB Glucose N.D. -0.5 N.D. -0.3 -0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 N.D. -0.4 -0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.6 N.D. -0.3 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +1.2 N.D. -2.0 -0.1
auxotrophy Dropout Complete N.D. -0.6 N.D. -0.2 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. -0.7 N.D. -0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.4 N.D. -0.2 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.8 N.D. +0.7 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +1.3 N.D. -2.0 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.3 N.D. -0.1 -0.7
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.4 N.D. -0.7 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. +0.1 -0.0
growth on oleic acid YNB Oleic Acid N.D. +0.9 N.D. -0.8 +0.1
growth on oleic acid YNB Glucose N.D. +0.5 N.D. -0.3 +0.0
growth on ricinoleic acid YNB Glucose N.D. +0.9 N.D. -0.8 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 N.D. +0.2 +0.4
growth on ypd YPD N.D. +0.6 N.D. +0.5 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +1.1 N.D. +0.2 +0.0
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