Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13926 and 13927 are separated by 86 nucleotides13927 and 13928 are separated by 80 nucleotides13928 and 13929 are separated by 689 nucleotides13929 and 13930 are separated by 200 nucleotides mRNA_5558: 13926 - KOG3328 HGG motif-containing thioesterase, at 376,866 to 377,685 13926 mRNA_5559: 13927 - KOG2406 MADS box transcription factor, at 377,772 to 380,881 13927 mRNA_5560: 13928 - BLAST PROTEIN D2013.6 [Ceraceosorus bombacis], at 380,962 to 381,944 13928 mRNA_5561: 13929 - Hypothetical Protein, at 382,634 to 383,572 13929 mRNA_5562: 13930 - KOG0257 Kynurenine aminotransferase, glutamine transaminase K, at 383,773 to 385,239 13930
Group Condition mRNA_5558 mRNA_5559 mRNA_5560 mRNA_5561 mRNA_5562
13926 13927 13928 13929 13930
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 N.D. -3.0 -2.2 -0.8
facs on lipid accumulation Sorted High BODIPY Gate -0.9 N.D. -1.5 -0.1 -0.1
growth on ypd YPD -0.3 N.D. -0.8 -0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.8 N.D. +0.3 -1.0 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. -0.9 -0.4 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.6 N.D. -0.2 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 N.D. +0.1 -0.1 -0.6
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. -0.5 -0.1 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. -0.6 -0.0 -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. -0.4 -0.1 -0.2
auxotrophy YNB Glucose -0.4 N.D. +0.4 -0.4 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.0 N.D. +0.2 -0.5 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.4 N.D. -0.2 -0.3 -0.2
growth on ricinoleic acid YNB Glucose +0.2 N.D. +0.0 -0.6 -0.0
growth on oleic acid YNB Oleic Acid +0.1 N.D. -0.4 -0.2 +0.3
auxotrophy Dropout Complete -0.2 N.D. +0.6 -0.3 -0.2
growth on oleic acid YNB Glucose +0.0 N.D. +0.2 -0.4 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.7 N.D. +0.8 +0.1 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. +0.4 +0.1 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. +0.5 -0.3 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 N.D. +1.0 +0.1 -0.4
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