Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13946 and 13947 are separated by 1210 nucleotides13947 and 13948 are separated by 791 nucleotides13948 and 13949 are separated by 760 nucleotides13949 and 13950 are separated by 366 nucleotides mRNA_5578: 13946 - HMMPfam-HMG (high mobility group) box-PF00505,ProSiteProfiles-HMG boxes A and B DNA-binding domains profile.-PS50118,SMART-high mobility group-SM00398,SUPERFAMILY--SSF47095, at 419,632 to 421,061 13946 mRNA_5579: 13947 - HMMPfam-Zinc-binding dehydrogenase-PF00107,HMMPfam-Alcohol dehydrogenase GroES-like domain-PF08240,SUPERFAMILY--SSF50129,SUPERFAMILY--SSF51735, at 422,272 to 423,518 13947 mRNA_5580: 13948 - K00162 PDHB, pdhB pyruvate dehydrogenase E1 component beta subunit, at 424,310 to 425,729 13948 mRNA_5581: 13949 - KOG1198 Zinc-binding oxidoreductase, at 426,490 to 430,753 13949 mRNA_5582: 13950 - KOG0254 Predicted transporter (major facilitator superfamily), at 431,120 to 432,974 13950
Group Condition mRNA_5578 mRNA_5579 mRNA_5580 mRNA_5581 mRNA_5582
13946 13947 13948 13949 13950
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 N.D. -0.5 -0.5
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.5 N.D. -0.4 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 N.D. +0.2 -0.7
growth on oleic acid YNB Oleic Acid N.D. +0.3 N.D. -0.1 -0.5
auxotrophy Dropout Complete N.D. -0.7 N.D. +0.4 -0.0
auxotrophy YNB Glucose N.D. -0.7 N.D. +0.5 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 N.D. +0.0 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 N.D. -0.1 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.5 N.D. -0.1 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.4 N.D. -0.0 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 N.D. +0.1 +0.2
growth on oleic acid YNB Glucose N.D. +0.5 N.D. -0.2 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.7 N.D. -0.5 -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.3 N.D. +0.3 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.3 N.D. -0.1 +0.1
growth on ricinoleic acid YNB Glucose N.D. -0.1 N.D. +0.0 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.5 N.D. +0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.4 N.D. +0.8 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 N.D. +0.3 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.6 N.D. +0.1 +0.1
growth on ypd YPD N.D. +0.5 N.D. +0.5 +0.3
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