Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13952 and 13953 are separated by 769 nucleotides13953 and 13954 are separated by 1032 nucleotides13954 and 13955 are separated by 234 nucleotides13955 and 13956 are separated by 410 nucleotides mRNA_5584: 13952 - K14780 DHX37, DHR1 ATP-dependent RNA helicase DHX37/DHR1, at 434,778 to 438,176 13952 mRNA_5585: 13953 - HMMPfam-PB1 domain-PF00564,SUPERFAMILY--SSF54277, at 438,946 to 439,812 13953 mRNA_5586: 13954 - HMMPfam-Response regulator receiver domain-PF00072,HMMPfam-Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase-PF02518,ProSiteProfiles-Response regulatory domain profile.-PS50110,SMART-cheY-homologous receiver domain-SM00448,SUPERFAMILY--SSF52172,SUPERFAMILY--SSF55874, at 440,845 to 442,225 13954 mRNA_5587: 13955 - KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily), at 442,460 to 444,934 13955 mRNA_5588: 13956 - K04646 CLTC clathrin heavy chain, at 445,345 to 451,026 13956
Group Condition mRNA_5584 mRNA_5585 mRNA_5586 mRNA_5587 mRNA_5588
13952 13953 13954 13955 13956
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 -0.4 -0.6 N.D.
auxotrophy YNB Glucose N.D. -0.1 -0.2 -0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.5 +0.0 +0.1 N.D.
auxotrophy Dropout Complete N.D. -0.0 -0.2 -0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 -0.0 -0.3 N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.5 -0.2 -0.5 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.4 -0.2 -0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.3 +0.1 +0.2 N.D.
growth on oleic acid YNB Glucose N.D. +0.3 -0.1 -0.3 N.D.
growth on oleic acid YNB Oleic Acid N.D. +0.2 +0.0 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.5 +0.0 +0.5 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 +0.1 +0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 +0.1 +0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 -0.2 +0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 +0.2 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 -0.0 +0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 +0.1 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 +0.1 +0.3 N.D.
growth on ypd YPD N.D. +0.2 -0.1 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 +0.1 +0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 +0.4 +0.3 N.D.
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