Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13955 and 13956 are separated by 410 nucleotides13956 and 13957 are separated by 1620 nucleotides13957 and 13958 are separated by 116 nucleotides13958 and 13959 are separated by 1071 nucleotides mRNA_5587: 13955 - KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily), at 442,460 to 444,934 13955 mRNA_5588: 13956 - K04646 CLTC clathrin heavy chain, at 445,345 to 451,026 13956 mRNA_5589: 13957 - HMMPfam-Region in Clathrin and VPS-PF00637,ProSiteProfiles-Clathrin heavy-chain (CHCR) repeat profile.-PS50236,SMART-Clathrin heavy chain repeat homology-SM00299,SUPERFAMILY--SSF48371, at 452,647 to 453,380 13957 mRNA_5590: 13958 - K11137 TELO2, TEL2 telomere length regulation protein, at 453,497 to 457,057 13958 mRNA_5591: 13959 - K11182 AOC1, ABP1 diamine oxidase, at 458,129 to 461,343 13959
Group Condition mRNA_5587 mRNA_5588 mRNA_5589 mRNA_5590 mRNA_5591
13955 13956 13957 13958 13959
growth on ricinoleic acid YNB Methylricinoleic Acid -0.6 N.D. -0.3 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. -0.8 N.D. -0.0
growth on ricinoleic acid YNB Glucose -0.5 N.D. -0.2 N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 N.D. -0.5 N.D. -0.4
growth on oleic acid YNB Oleic Acid -0.2 N.D. -0.3 N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. -0.3 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. +0.2 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 N.D. +0.3 N.D. +0.3
growth on oleic acid YNB Glucose -0.3 N.D. +0.5 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 N.D. +0.1 N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. +0.1 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. +0.2 N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. +0.6 N.D. +0.2
auxotrophy YNB Glucose -0.1 N.D. +0.5 N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 N.D. +0.1 N.D. +0.1
auxotrophy Dropout Complete -0.1 N.D. +0.7 N.D. +0.0
growth on ypd YPD +0.3 N.D. +0.1 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. +0.5 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 N.D. +0.4 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. +0.9 N.D. -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. +1.0 N.D. +0.4
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