Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13957 and 13958 are separated by 116 nucleotides13958 and 13959 are separated by 1071 nucleotides13959 and 13960 are separated by 255 nucleotides13960 and 13961 are separated by 63 nucleotides mRNA_5589: 13957 - HMMPfam-Region in Clathrin and VPS-PF00637,ProSiteProfiles-Clathrin heavy-chain (CHCR) repeat profile.-PS50236,SMART-Clathrin heavy chain repeat homology-SM00299,SUPERFAMILY--SSF48371, at 452,647 to 453,380 13957 mRNA_5590: 13958 - K11137 TELO2, TEL2 telomere length regulation protein, at 453,497 to 457,057 13958 mRNA_5591: 13959 - K11182 AOC1, ABP1 diamine oxidase, at 458,129 to 461,343 13959 mRNA_5592: 13960 - HMMPfam-Alcohol acetyltransferase-PF07247,SUPERFAMILY--SSF52777, at 461,599 to 463,064 13960 mRNA_5593: 13961 - K00905 BCKDK [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] kinase, at 463,128 to 464,888 13961
Group Condition mRNA_5589 mRNA_5590 mRNA_5591 mRNA_5592 mRNA_5593
13957 13958 13959 13960 13961
growth on ricinoleic acid YNB Glucose -0.2 N.D. +0.1 -0.8 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 N.D. +0.1 -0.4 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.8 N.D. -0.0 +0.4 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 N.D. -0.4 +0.6 -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. -0.1 -0.4 -0.0
growth on oleic acid YNB Oleic Acid -0.3 N.D. +0.1 +0.0 -0.1
growth on oleic acid YNB Glucose +0.5 N.D. +0.1 -0.7 +0.1
auxotrophy YNB Glucose +0.5 N.D. +0.0 -0.5 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. +0.3 -0.5 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. -0.2 +0.2 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 N.D. +0.1 +0.4 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. +0.1 +0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.6 N.D. +0.2 -0.3 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.9 N.D. -0.0 -0.4 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.5 N.D. -0.1 -0.0 +0.2
auxotrophy Dropout Complete +0.7 N.D. +0.0 +0.1 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. +0.1 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 N.D. +0.2 +0.2 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +1.0 N.D. +0.4 -0.2 -0.3
growth on ypd YPD +0.1 N.D. +0.2 +0.5 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. +0.1 +0.7 +0.1
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