Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13961 and 13962 are separated by 261 nucleotides13962 and 13963 are separated by 273 nucleotides13963 and 13964 are separated by 1572 nucleotides13964 and 13965 are separated by 878 nucleotides mRNA_5593: 13961 - K00905 BCKDK [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] kinase, at 463,128 to 464,888 13961 mRNA_5594: 13962 - K07943 ARL2 ADP-ribosylation factor-like protein 2, at 465,150 to 466,141 13962 mRNA_5595: 13963 - K11294 NCL, NSR1 nucleolin, at 466,415 to 467,468 13963 mRNA_5596: 13964 - KOG2806 Chitinase, at 469,041 to 471,762 13964 mRNA_5597: 13965 - Hypothetical Protein, at 472,641 to 473,822 13965
Group Condition mRNA_5593 mRNA_5594 mRNA_5595 mRNA_5596 mRNA_5597
13961 13962 13963 13964 13965
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -1.1 N.D. -0.7 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -1.3 N.D. -0.2 +0.3
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.6 N.D. -0.2 -0.5
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -1.2 N.D. +0.4 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.6 N.D. -0.1 -0.4
growth on oleic acid YNB Glucose +0.1 -0.2 N.D. -0.3 -0.5
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.8 N.D. -0.0 -0.2
growth on oleic acid YNB Oleic Acid -0.1 -0.5 N.D. +0.1 -0.3
auxotrophy Dropout Complete -0.0 -0.3 N.D. +0.3 -0.5
auxotrophy YNB Glucose -0.0 -0.4 N.D. +0.2 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.3 N.D. -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.6 N.D. -0.5 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 +0.2 N.D. -0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.2 N.D. +0.2 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 +0.1 N.D. +0.2 -0.4
ypd buoyancy 40 hrs High Buoyancy +0.2 +1.0 N.D. -0.6 -0.2
growth on ypd YPD +0.2 -0.1 N.D. +0.7 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.3 N.D. +0.5 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.3 N.D. +0.3 +0.6
growth on ricinoleic acid YNB Glucose -0.1 +1.1 N.D. +0.3 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 +1.3 N.D. +0.7 +0.1
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