Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14001 and 14002 are separated by 322 nucleotides14002 and 14003 are separated by 595 nucleotides14003 and 14004 are separated by 273 nucleotides14004 and 14005 are separated by 290 nucleotides mRNA_5633: 14001 - K01679 E4.2.1.2B, fumC fumarate hydratase, class II, at 558,284 to 560,290 14001 mRNA_5634: 14002 - K00750 GYG1, GYG2 glycogenin, at 560,613 to 563,450 14002 mRNA_5635: 14003 - HMMPfam-Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y-PF15159, at 564,046 to 564,667 14003 mRNA_5636: 14004 - BLAST Regulator of phospholipase D SRF1 [Hypsizygus marmoreus], at 564,941 to 566,584 14004 mRNA_5637: 14005 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 566,875 to 570,624 14005
Group Condition mRNA_5633 mRNA_5634 mRNA_5635 mRNA_5636 mRNA_5637
14001 14002 14003 14004 14005
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -1.0 N.D. -0.9 -0.6
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -1.3 N.D. -1.0 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -1.3 N.D. -0.3 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.3 N.D. -0.9 +0.0
growth on oleic acid YNB Oleic Acid N.D. -0.8 N.D. +0.0 -0.2
growth on ypd YPD N.D. -0.3 N.D. -0.4 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 N.D. -0.6 -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 N.D. -0.5 -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 N.D. -0.1 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 N.D. -0.2 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.0 N.D. -0.2 -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 N.D. -0.5 +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 N.D. -0.6 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.5 N.D. +0.1 +0.3
growth on ricinoleic acid YNB Glucose N.D. +0.3 N.D. -0.2 -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 N.D. -0.3 +0.2
auxotrophy Dropout Complete N.D. +0.1 N.D. -0.3 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 N.D. -0.0 -0.0
growth on oleic acid YNB Glucose N.D. +0.3 N.D. +0.0 -0.1
auxotrophy YNB Glucose N.D. +0.2 N.D. -0.5 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 N.D. +0.3 +0.2
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