Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14015 and 14016 are separated by 186 nucleotides14016 and 14017 are separated by 688 nucleotides14017 and 14018 are separated by 474 nucleotides14018 and 14019 are separated by 253 nucleotides mRNA_5647: 14015 - KOG4719 Nuclear pore complex protein, at 588,479 to 592,654 14015 mRNA_5648: 14016 - K11268 ESCO, ECO1 N-acetyltransferase, at 592,841 to 594,405 14016 mRNA_5649: 14017 - SUPERFAMILY--SSF52047, at 595,094 to 596,616 14017 mRNA_5650: 14018 - Hypothetical Protein, at 597,091 to 598,437 14018 mRNA_5651: 14019 - K13728 MAD2L2 mitotic spindle assembly checkpoint protein MAD2B, at 598,691 to 599,963 14019
Group Condition mRNA_5647 mRNA_5648 mRNA_5649 mRNA_5650 mRNA_5651
14015 14016 14017 14018 14019
facs on lipid accumulation Sorted High BODIPY Gate +0.5 N.D. -0.3 -0.7 -0.6
auxotrophy YNB Glucose -0.1 N.D. +0.1 -0.8 +0.1
auxotrophy Dropout Complete -0.3 N.D. +0.3 -0.8 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. -0.3 +0.3 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 N.D. -0.1 -0.2 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. +0.2 -0.2 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. -0.3 +0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. +0.5 -0.5 -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.3 N.D. -0.1 -0.1 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 N.D. -0.2 +0.1 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. -0.3 -0.0 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. +0.0 -0.0 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.3 N.D. -0.1 +0.3 +0.1
growth on oleic acid YNB Glucose -0.1 N.D. +0.1 -0.1 +0.3
growth on ypd YPD -0.1 N.D. -0.4 +0.2 +0.7
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 N.D. -0.2 +0.5 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. -0.4 +0.0 +1.2
growth on oleic acid YNB Oleic Acid +0.2 N.D. +0.1 +0.4 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. -0.1 +0.8 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.8 N.D. +0.1 -0.1 -0.1
growth on ricinoleic acid YNB Glucose -0.2 N.D. -0.2 -0.1 +1.6
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