Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14019 and 14020 are separated by 2169 nucleotides14020 and 14021 are separated by 921 nucleotides14021 and 14022 are separated by 599 nucleotides14022 and 14023 are separated by 216 nucleotides mRNA_5651: 14019 - K13728 MAD2L2 mitotic spindle assembly checkpoint protein MAD2B, at 598,691 to 599,963 14019 mRNA_5652: 14020 - KOG4443 Putative transcription factor HALR/MLL3, involved in embryonic development, at 602,133 to 602,528 14020 mRNA_5653: 14021 - Hypothetical Protein, at 603,450 to 604,586 14021 mRNA_5654: 14022 - K01637 E4.1.3.1, aceA isocitrate lyase, at 605,186 to 607,588 14022 mRNA_5655: 14023 - K01115 PLD1_2 phospholipase D1/2, at 607,805 to 615,263 14023
Group Condition mRNA_5651 mRNA_5652 mRNA_5653 mRNA_5654 mRNA_5655
14019 14020 14021 14022 14023
growth on oleic acid YNB Oleic Acid +0.1 -1.0 -0.0 -3.5 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +1.7 -0.9 -4.3 -0.7
growth on ricinoleic acid YNB Methylricinoleic Acid +1.2 +1.1 -0.5 -4.6 -0.4
auxotrophy YNB Glucose 75mM Methionine +0.0 -1.0 -0.7 +0.0 -0.5
auxotrophy YNB Glucose +0.1 -0.8 -0.5 -0.1 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -1.0 +0.1 +0.1 -0.9
auxotrophy Dropout Complete +0.0 -0.5 -0.4 -0.2 -0.4
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.9 -0.1 +0.1 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 -0.7 +0.5 -0.1 +0.2
growth on oleic acid YNB Glucose +0.3 -0.1 +0.0 -0.1 -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.5 +0.4 +0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.5 +0.3 +0.0 +0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.7 +0.1 -0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.3 -0.2 +0.4 +0.1 -0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.6 +0.6 +0.6 +0.1 +0.1
growth on ypd YPD +0.7 +0.3 -0.2 +0.0 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.9 +0.5 -0.5 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +1.5 +0.2 -0.3 -0.1
growth on ricinoleic acid YNB Glucose +1.6 +1.2 -0.5 -0.3 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +2.7 +0.5 -0.5 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 +3.2 +1.4 +0.1 +0.2
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