Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14032 and 14033 are separated by 1489 nucleotides14033 and 14034 are separated by 386 nucleotides14034 and 14035 are separated by 4208 nucleotides14035 and 14036 are separated by 193 nucleotides mRNA_5664: 14032 - K01262 pepP Xaa-Pro aminopeptidase, at 630,515 to 632,715 14032 mRNA_5665: 14033 - K15083 RAD16 DNA repair protein RAD16, at 634,205 to 636,873 14033 mRNA_5666: 14034 - Hypothetical Protein, at 637,260 to 638,584 14034 mRNA_5667: 14035 - K11233 SSK1 osomolarity two-component system, response regulator SSK1, at 642,793 to 648,199 14035 mRNA_5668: 14036 - K01227 E3.2.1.96 mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase, at 648,393 to 650,754 14036
Group Condition mRNA_5664 mRNA_5665 mRNA_5666 mRNA_5667 mRNA_5668
14032 14033 14034 14035 14036
facs on lipid accumulation Sorted Low BODIPY Gate -1.0 -0.1 -0.2 -0.6 -0.6
facs on lipid accumulation Sorted High BODIPY Gate -1.4 +0.1 +0.1 -0.5 -0.4
facs on lipid accumulation Sorted No BODIPY Gate -1.3 +0.1 -0.2 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.6 -0.1 +0.1 -0.0 -0.5
growth on ricinoleic acid YNB Glucose -0.1 -0.1 -0.0 -0.3 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 -0.2 +0.2 -0.3 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 -0.2 +0.2 -0.4 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 -0.1 -0.2 -0.1 -0.3
auxotrophy Dropout Complete +0.7 +0.0 -0.1 -1.3 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.2 +0.0 -0.6 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 +0.1 +0.1 -0.5 -0.2
growth on oleic acid YNB Oleic Acid -0.2 -0.2 +0.1 -0.3 +0.1
auxotrophy YNB Glucose +0.4 -0.2 +0.3 -0.7 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.0 +0.1 -0.6 -0.0
growth on ypd YPD -0.1 -0.3 +0.2 +0.2 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 -0.0 -0.1 -0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.2 -0.2 -0.0 -0.0
growth on oleic acid YNB Glucose +0.1 -0.1 +0.4 -0.2 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.5 +0.1 -0.3 +0.5 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 -0.5 -0.4 +1.2 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.7 +0.0 -0.1 +0.3 +0.0
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