Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14033 and 14034 are separated by 386 nucleotides14034 and 14035 are separated by 4208 nucleotides14035 and 14036 are separated by 193 nucleotides14036 and 14037 are separated by 360 nucleotides mRNA_5665: 14033 - K15083 RAD16 DNA repair protein RAD16, at 634,205 to 636,873 14033 mRNA_5666: 14034 - Hypothetical Protein, at 637,260 to 638,584 14034 mRNA_5667: 14035 - K11233 SSK1 osomolarity two-component system, response regulator SSK1, at 642,793 to 648,199 14035 mRNA_5668: 14036 - K01227 E3.2.1.96 mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase, at 648,393 to 650,754 14036 mRNA_5669: 14037 - K01736 aroC chorismate synthase, at 651,115 to 652,658 14037
Group Condition mRNA_5665 mRNA_5666 mRNA_5667 mRNA_5668 mRNA_5669
14033 14034 14035 14036 14037
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.2 -0.6 -0.6 N.D.
auxotrophy Dropout Complete +0.0 -0.1 -1.3 -0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.0 -0.6 -0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.2 -0.4 -0.4 N.D.
growth on ricinoleic acid YNB Glucose -0.1 -0.0 -0.3 -0.4 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.2 -0.1 -0.3 N.D.
auxotrophy YNB Glucose -0.2 +0.3 -0.7 -0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.1 -0.5 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 +0.2 -0.3 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.1 -0.5 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.1 -0.0 -0.5 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.0 +0.1 -0.6 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 -0.1 -0.1 -0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.2 -0.1 -0.1 N.D.
growth on oleic acid YNB Oleic Acid -0.2 +0.1 -0.3 +0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.2 -0.2 -0.0 -0.0 N.D.
growth on ypd YPD -0.3 +0.2 +0.2 -0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.1 +0.3 +0.0 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.3 +0.5 -0.0 N.D.
growth on oleic acid YNB Glucose -0.1 +0.4 -0.2 +0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 -0.4 +1.2 +0.1 N.D.
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