Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14037 and 14038 are separated by 396 nucleotides14038 and 14039 are separated by 853 nucleotides14039 and 14040 are separated by 163 nucleotides14040 and 14041 are separated by 777 nucleotides mRNA_5669: 14037 - K01736 aroC chorismate synthase, at 651,115 to 652,658 14037 mRNA_5670: 14038 - K18995 DHX29 ATP-dependent RNA helicase DHX29, at 653,055 to 658,546 14038 mRNA_5671: 14039 - K11675 IES1 Ino eighty subunit 1, at 659,400 to 661,417 14039 mRNA_5672: 14040 - HMMPfam-Putative methyltransferase-PF10294,SUPERFAMILY--SSF53335, at 661,581 to 662,502 14040 mRNA_5673: 14041 - Hypothetical Protein, at 663,280 to 665,489 14041
Group Condition mRNA_5669 mRNA_5670 mRNA_5671 mRNA_5672 mRNA_5673
14037 14038 14039 14040 14041
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.5 -0.2 N.D. -0.0
auxotrophy Dropout Complete N.D. +0.1 -0.7 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 -0.4 N.D. -0.1
auxotrophy YNB Glucose N.D. -0.0 -0.6 N.D. +0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.4 +0.0 N.D. -0.1
growth on ricinoleic acid YNB Glucose N.D. -0.1 -0.1 N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 -0.6 N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 -0.3 N.D. +0.0
growth on oleic acid YNB Oleic Acid N.D. +0.1 +0.0 N.D. -0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 -0.2 N.D. +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 -0.1 N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 +0.1 N.D. +0.0
growth on oleic acid YNB Glucose N.D. +0.0 +0.2 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 +0.1 N.D. +0.2
growth on ypd YPD N.D. -0.1 +0.0 N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 -0.1 N.D. +0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 +0.1 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 +0.5 N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.3 +0.6 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 +1.0 N.D. +0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.0 +0.9 N.D. +0.8
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