Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14047 and 14048 are separated by 223 nucleotides14048 and 14049 are separated by 390 nucleotides14049 and 14050 are separated by 87 nucleotides14050 and 14051 are separated by 1080 nucleotides mRNA_5679: 14047 - KOG1944 Peroxisomal membrane protein MPV17 and related proteins, at 675,766 to 676,852 14047 mRNA_5680: 14048 - Hypothetical Protein, at 677,076 to 678,500 14048 mRNA_5681: 14049 - KOG3146 Dolichyl pyrophosphate phosphatase and related acid phosphatases, at 678,891 to 679,763 14049 mRNA_5682: 14050 - K03349 APC2 anaphase-promoting complex subunit 2, at 679,851 to 681,974 14050 mRNA_5683: 14051 - K15505 RAD5 DNA repair protein RAD5, at 683,055 to 687,923 14051
Group Condition mRNA_5679 mRNA_5680 mRNA_5681 mRNA_5682 mRNA_5683
14047 14048 14049 14050 14051
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.1 N.D. -4.7 +0.3
auxotrophy YNB Glucose 75mM Methionine +0.7 -0.2 N.D. -2.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.4 -0.1 N.D. -1.9 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 +0.1 N.D. -2.0 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.1 N.D. -2.1 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -0.1 N.D. -1.7 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 +0.1 N.D. -2.0 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 +0.0 N.D. -1.3 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +0.1 N.D. -1.5 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.3 N.D. -1.0 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.0 +0.3 N.D. -1.2 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.7 +0.0 N.D. -1.4 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 +0.4 N.D. -0.4 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.5 +0.1 N.D. -0.7 +0.1
growth on oleic acid YNB Glucose +0.4 -0.4 N.D. -0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.5 +0.1 N.D. -0.4 -0.1
growth on oleic acid YNB Oleic Acid +0.2 +0.1 N.D. -0.2 -0.0
auxotrophy YNB Glucose +0.6 -0.1 N.D. -0.4 -0.1
growth on ricinoleic acid YNB Glucose -0.1 -0.1 N.D. +0.2 +0.0
auxotrophy Dropout Complete +0.7 -0.1 N.D. -0.2 -0.1
growth on ypd YPD +0.5 +0.3 N.D. -0.8 +0.3
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