Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14050 and 14051 are separated by 1080 nucleotides14051 and 14052 are separated by 74 nucleotides14052 and 14053 are separated by 108 nucleotides14053 and 14054 are separated by 139 nucleotides mRNA_5682: 14050 - K03349 APC2 anaphase-promoting complex subunit 2, at 679,851 to 681,974 14050 mRNA_5683: 14051 - K15505 RAD5 DNA repair protein RAD5, at 683,055 to 687,923 14051 mRNA_5684: 14052 - K07404 pgl 6-phosphogluconolactonase, at 687,998 to 689,292 14052 mRNA_5685: 14053 - HMMPfam-Complex 1 protein (LYR family)-PF05347, at 689,401 to 690,702 14053 mRNA_5686: 14054 - K05294 PGAP1 glycosylphosphatidylinositol deacylase, at 690,842 to 694,591 14054
Group Condition mRNA_5682 mRNA_5683 mRNA_5684 mRNA_5685 mRNA_5686
14050 14051 14052 14053 14054
facs on lipid accumulation Sorted Low BODIPY Gate -4.7 +0.3 -0.2 N.D. -0.6
growth on ricinoleic acid YNB Ricinoleic Acid -2.0 +0.2 -0.1 N.D. -1.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -1.7 +0.2 +0.1 N.D. -1.2
auxotrophy YNB Glucose 75mM Methionine -2.2 -0.1 +0.0 N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -1.4 +0.1 -0.1 N.D. -0.4
ypd buoyancy 40 hrs Low Buoyancy -1.9 -0.1 -0.1 N.D. +0.4
facs on lipid accumulation Sorted High BODIPY Gate -1.5 +0.1 -0.1 N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -2.1 +0.4 -0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -2.0 +0.2 +0.1 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate -1.2 +0.2 -0.1 N.D. -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.3 +0.2 -0.1 N.D. -0.4
growth on ypd YPD -0.8 +0.3 -0.2 N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.7 +0.1 -0.0 N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine -1.0 +0.1 +0.0 N.D. +0.3
ypd buoyancy 40 hrs High Buoyancy -0.4 -0.1 -0.1 N.D. +0.1
growth on oleic acid YNB Oleic Acid -0.2 -0.0 +0.1 N.D. +0.0
auxotrophy YNB Glucose -0.4 -0.1 +0.2 N.D. +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 +0.1 -0.1 N.D. +0.6
auxotrophy Dropout Complete -0.2 -0.1 +0.1 N.D. +0.6
growth on ricinoleic acid YNB Glucose +0.2 +0.0 -0.1 N.D. +0.2
growth on oleic acid YNB Glucose -0.1 +0.1 +0.2 N.D. +0.7
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