Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14055 and 14056 are separated by 269 nucleotides14056 and 14057 are separated by 121 nucleotides14057 and 14058 are separated by 279 nucleotides14058 and 14059 are separated by 67 nucleotides mRNA_5687: 14055 - K08764 SCP2, SCPX sterol carrier protein 2, at 694,932 to 696,791 14055 mRNA_5688: 14056 - K12199 VTA1, LIP5 vacuolar protein sorting-associated protein VTA1, at 697,061 to 698,504 14056 mRNA_5689: 14057 - KOG4206 Spliceosomal protein snRNP-U1A/U2B, at 698,626 to 699,781 14057 mRNA_5690: 14058 - K17710 PTCD1 pentatricopeptide repeat domain-containing protein 1, at 700,061 to 702,418 14058 mRNA_5691: 14059 - K05956 RABGGTB geranylgeranyl transferase type-2 subunit beta, at 702,486 to 703,817 14059
Group Condition mRNA_5687 mRNA_5688 mRNA_5689 mRNA_5690 mRNA_5691
14055 14056 14057 14058 14059
facs on lipid accumulation Sorted Low BODIPY Gate -1.1 -3.9 -1.9 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -2.1 -1.3 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.5 -1.0 -0.6 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 -0.9 -0.6 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.7 -0.6 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 -0.0 -0.3 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 +0.5 -0.5 N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.3 -0.2 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 +0.0 -0.1 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.2 -0.4 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 +0.1 +0.1 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.3 +0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 -0.4 +0.9 N.D. N.D.
auxotrophy Dropout Complete -0.3 -0.3 +0.7 N.D. N.D.
growth on ypd YPD -0.2 +0.7 -0.2 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.6 -0.0 N.D. N.D.
growth on oleic acid YNB Glucose -0.0 -0.2 +0.6 N.D. N.D.
auxotrophy YNB Glucose +0.3 +0.6 -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +1.2 +0.5 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +1.1 +0.6 N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.2 +1.5 +0.7 N.D. N.D.
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