Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14056 and 14057 are separated by 121 nucleotides14057 and 14058 are separated by 279 nucleotides14058 and 14059 are separated by 67 nucleotides14059 and 14060 are separated by 113 nucleotides mRNA_5688: 14056 - K12199 VTA1, LIP5 vacuolar protein sorting-associated protein VTA1, at 697,061 to 698,504 14056 mRNA_5689: 14057 - KOG4206 Spliceosomal protein snRNP-U1A/U2B, at 698,626 to 699,781 14057 mRNA_5690: 14058 - K17710 PTCD1 pentatricopeptide repeat domain-containing protein 1, at 700,061 to 702,418 14058 mRNA_5691: 14059 - K05956 RABGGTB geranylgeranyl transferase type-2 subunit beta, at 702,486 to 703,817 14059 mRNA_5692: 14060 - K07342 SEC61G, SSS1, secE protein transport protein SEC61 subunit gamma and related proteins, at 703,931 to 704,550 14060
Group Condition mRNA_5688 mRNA_5689 mRNA_5690 mRNA_5691 mRNA_5692
14056 14057 14058 14059 14060
facs on lipid accumulation Sorted Low BODIPY Gate -3.9 -1.9 N.D. N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -2.1 -1.3 N.D. N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -1.0 -0.6 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.9 -0.6 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.7 -0.6 N.D. N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy -0.0 -0.3 N.D. N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.2 -0.4 N.D. N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.2 +0.1 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 -0.1 N.D. N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 -0.5 N.D. N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -0.3 +0.3 N.D. N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.1 N.D. N.D. N.D.
auxotrophy Dropout Complete -0.3 +0.7 N.D. N.D. N.D.
growth on oleic acid YNB Glucose -0.2 +0.6 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 +0.9 N.D. N.D. N.D.
growth on ypd YPD +0.7 -0.2 N.D. N.D. N.D.
auxotrophy YNB Glucose +0.6 -0.1 N.D. N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.6 -0.0 N.D. N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +1.1 +0.6 N.D. N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.2 +0.5 N.D. N.D. N.D.
growth on ricinoleic acid YNB Glucose +1.5 +0.7 N.D. N.D. N.D.
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