Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14058 and 14059 are separated by 67 nucleotides14059 and 14060 are separated by 113 nucleotides14060 and 14061 are separated by 343 nucleotides14061 and 14062 are separated by 244 nucleotides mRNA_5690: 14058 - K17710 PTCD1 pentatricopeptide repeat domain-containing protein 1, at 700,061 to 702,418 14058 mRNA_5691: 14059 - K05956 RABGGTB geranylgeranyl transferase type-2 subunit beta, at 702,486 to 703,817 14059 mRNA_5692: 14060 - K07342 SEC61G, SSS1, secE protein transport protein SEC61 subunit gamma and related proteins, at 703,931 to 704,550 14060 mRNA_5693: 14061 - KOG4186 Peroxisomal biogenesis protein (peroxin), at 704,894 to 706,050 14061 mRNA_5694: 14062 - HMMPfam-Pyridoxamine 5'-phosphate oxidase-PF13883,SUPERFAMILY--SSF50475, at 706,295 to 707,436 14062
Group Condition mRNA_5690 mRNA_5691 mRNA_5692 mRNA_5693 mRNA_5694
14058 14059 14060 14061 14062
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. -3.7 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. -3.9 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. -2.7 +0.0
growth on ypd YPD N.D. N.D. N.D. -1.3 +0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -1.2 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. -0.3 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.5 -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. -0.6 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. -0.3 -0.2
growth on oleic acid YNB Glucose N.D. N.D. N.D. -0.3 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.3 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. -0.3 +0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. -0.2 +0.2
auxotrophy YNB Glucose N.D. N.D. N.D. -0.2 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. -0.4 +0.4
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. -0.2 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.2 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. +0.3 -0.1
auxotrophy Dropout Complete N.D. N.D. N.D. -0.0 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. +0.1 +0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. +0.1 +0.4
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