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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
14059 and 14060 are separated by 113 nucleotides
14060 and 14061 are separated by 343 nucleotides
14061 and 14062 are separated by 244 nucleotides
14062 and 14063 are separated by 624 nucleotides
mRNA_5691: 14059 - K05956 RABGGTB geranylgeranyl transferase type-2 subunit beta, at 702,486 to 703,817
14059
mRNA_5692: 14060 - K07342 SEC61G, SSS1, secE protein transport protein SEC61 subunit gamma and related proteins, at 703,931 to 704,550
14060
mRNA_5693: 14061 - KOG4186 Peroxisomal biogenesis protein (peroxin), at 704,894 to 706,050
14061
mRNA_5694: 14062 - HMMPfam-Pyridoxamine 5'-phosphate oxidase-PF13883,SUPERFAMILY--SSF50475, at 706,295 to 707,436
14062
mRNA_5695: 14063 - K08869 ADCK, ABC1 aarF domain-containing kinase, at 708,061 to 710,208
14063
Group
Condition
mRNA
_5691
mRNA
_5692
mRNA
_5693
mRNA
_5694
mRNA
_5695
14059
14060
14061
14062
14063
growth on ricinoleic acid
YNB Ricinoleic Acid
N.D.
N.D.
-3.9
+0.2
-1.7
growth on oleic acid
YNB Oleic Acid
N.D.
N.D.
-3.7
-0.1
-1.0
growth on ricinoleic acid
YNB Methylricinoleic Acid
N.D.
N.D.
-2.7
+0.0
-1.6
facs on lipid accumulation
Sorted Low BODIPY Gate
N.D.
N.D.
-0.5
-0.0
-1.5
ypd buoyancy
40 hrs No Buoyancy Separation
N.D.
N.D.
-0.3
+0.1
-1.1
growth on ypd
YPD
N.D.
N.D.
-1.3
+0.0
+0.1
ypd buoyancy
40 hrs High Buoyancy
N.D.
N.D.
-0.4
+0.4
-1.1
auxotrophy
YNB Glucose
N.D.
N.D.
-0.2
+0.2
-1.0
buoyancy on lipid accumulation
40 hrs Median Buoyancy
N.D.
N.D.
-0.3
-0.3
-0.5
growth on oleic acid
YNB Glucose
N.D.
N.D.
-0.3
-0.2
-0.6
facs on lipid accumulation
Sorted High BODIPY Gate
N.D.
N.D.
-1.2
+0.3
-0.0
auxotrophy
YNB Glucose 75mM Arginine
N.D.
N.D.
-0.2
+0.2
-0.7
auxotrophy
YNB Glucose 75mM Methionine
N.D.
N.D.
-0.6
+0.1
-0.1
ypd buoyancy
40 hrs Low Buoyancy
N.D.
N.D.
+0.1
+0.1
-0.6
growth on ricinoleic acid
YNB Glucose
N.D.
N.D.
+0.1
+0.4
-0.8
ypd buoyancy
40 hrs Median Buoyancy
N.D.
N.D.
-0.3
+0.3
+0.0
facs on lipid accumulation
Sorted No BODIPY Gate
N.D.
N.D.
-0.2
+0.2
+0.0
buoyancy on lipid accumulation
40 hrs High Buoyancy
N.D.
N.D.
-0.3
-0.2
+0.4
auxotrophy
Dropout Complete
N.D.
N.D.
-0.0
+0.2
-0.2
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
N.D.
N.D.
+0.2
-0.2
-0.0
buoyancy on lipid accumulation
40 hrs Low Buoyancy
N.D.
N.D.
+0.3
-0.1
-0.1
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