Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14067 and 14068 are separated by 357 nucleotides14068 and 14069 are separated by 103 nucleotides14069 and 14070 are separated by 279 nucleotides14070 and 14071 are separated by 832 nucleotides mRNA_5699: 14067 - HMMPfam-Vault protein inter-alpha-trypsin domain-PF08487,HMMPfam-von Willebrand factor type A domain-PF13768,ProSiteProfiles-VWFA domain profile.-PS50234,ProSiteProfiles-VIT domain profile.-PS51468,SMART-von Willebrand factor (vWF) type A domain-SM00327,SMART-Vault protein Inter-alpha-Trypsin domain-SM00609,SUPERFAMILY--SSF53300, at 716,086 to 718,925 14067 mRNA_5700: 14068 - KOG0143 Iron/ascorbate family oxidoreductases, at 719,283 to 720,938 14068 mRNA_5701: 14069 - K13120 FAM32A protein FAM32A, at 721,042 to 721,632 14069 mRNA_5702: 14070 - K09478 ACADSB short/branched chain acyl-CoA dehydrogenase, at 721,912 to 723,848 14070 mRNA_5703: 14071 - KOG1289 Amino acid transporters, at 724,681 to 726,988 14071
Group Condition mRNA_5699 mRNA_5700 mRNA_5701 mRNA_5702 mRNA_5703
14067 14068 14069 14070 14071
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.2 -1.6 -3.0 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 -0.1 -1.6 -2.1 +0.3
growth on oleic acid YNB Oleic Acid -0.0 +0.1 +0.0 -2.0 +0.1
growth on ricinoleic acid YNB Glucose -0.0 +0.4 -1.6 -0.2 +0.1
auxotrophy Dropout Complete +0.3 -0.1 -0.8 -0.7 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 +0.3 -1.3 +0.1 -0.0
auxotrophy YNB Glucose +0.4 -0.0 -1.7 -0.1 +0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.2 -1.2 -0.1 +0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.1 -0.5 -0.4 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.1 -0.9 +0.6 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +0.1 -0.2 +0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 -0.2 -0.3 +0.6 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.3 -1.1 -0.0 +0.4
auxotrophy YNB Glucose 75mM Arginine +0.3 -0.1 -0.6 -0.1 +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.1 +0.1 -0.3 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.2 -0.1 -0.4 +0.4
growth on ypd YPD +0.3 -0.0 +0.2 -0.1 -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.0 +0.6 -0.1 +0.1
growth on oleic acid YNB Glucose +0.2 +0.1 +0.5 +0.0 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.4 +0.1 +0.2 +0.3 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.1 +0.9 +0.7 -0.2
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