Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14078 and 14079 are separated by 202 nucleotides14079 and 14080 are separated by 257 nucleotides14080 and 14081 are separated by 207 nucleotides14081 and 14082 are separated by 76 nucleotides mRNA_5710: 14078 - K03963 NDUFB7 NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 7, at 747,522 to 748,410 14078 mRNA_5711: 14079 - KOG4654 Uncharacterized conserved protein, at 748,613 to 751,704 14079 mRNA_5712: 14080 - K03253 EIF3B translation initiation factor 3 subunit B, at 751,962 to 754,544 14080 mRNA_5713: 14081 - K18819 GOLS inositol 3-alpha-galactosyltransferase, at 754,752 to 755,900 14081 mRNA_5714: 14082 - K01409 KAE1, tsaD, QRI7 N6-L-threonylcarbamoyladenine synthase, at 755,977 to 757,736 14082
Group Condition mRNA_5710 mRNA_5711 mRNA_5712 mRNA_5713 mRNA_5714
14078 14079 14080 14081 14082
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.0 N.D. -1.4 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.4 N.D. -0.7 N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.1 N.D. -0.8 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 N.D. -1.0 N.D.
growth on oleic acid YNB Glucose N.D. +0.0 N.D. -0.5 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 N.D. -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. -0.1 N.D.
growth on ypd YPD N.D. +0.1 N.D. -0.3 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.3 N.D. +0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 N.D. +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 N.D. -0.0 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.0 N.D. +0.4 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 N.D. +0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -0.0 N.D. +0.4 N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.2 N.D. +0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.4 N.D. +1.0 N.D.
auxotrophy YNB Glucose N.D. +0.1 N.D. +0.6 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 N.D. +0.5 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 N.D. +1.0 N.D.
auxotrophy Dropout Complete N.D. +0.3 N.D. +0.6 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.3 N.D. +1.3 N.D.
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