Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14080 and 14081 are separated by 207 nucleotides14081 and 14082 are separated by 76 nucleotides14082 and 14083 are separated by 90 nucleotides14083 and 14084 are separated by 501 nucleotides mRNA_5712: 14080 - K03253 EIF3B translation initiation factor 3 subunit B, at 751,962 to 754,544 14080 mRNA_5713: 14081 - K18819 GOLS inositol 3-alpha-galactosyltransferase, at 754,752 to 755,900 14081 mRNA_5714: 14082 - K01409 KAE1, tsaD, QRI7 N6-L-threonylcarbamoyladenine synthase, at 755,977 to 757,736 14082 mRNA_5715: 14083 - BLAST Elongator complex protein 4 [Ceraceosorus bombacis], at 757,827 to 758,501 14083 mRNA_5716: 14084 - Hypothetical Protein, at 759,003 to 760,646 14084
Group Condition mRNA_5712 mRNA_5713 mRNA_5714 mRNA_5715 mRNA_5716
14080 14081 14082 14083 14084
facs on lipid accumulation Sorted High BODIPY Gate N.D. -1.4 N.D. +0.3 +0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. -1.0 N.D. +0.2 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.7 N.D. +0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. -0.0 -0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.2 N.D. +0.2 -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 N.D. -0.3 -0.1
growth on ypd YPD N.D. -0.3 N.D. +0.1 +0.1
growth on oleic acid YNB Oleic Acid N.D. -0.8 N.D. +0.7 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.0 N.D. +0.2 -0.2
growth on oleic acid YNB Glucose N.D. -0.5 N.D. +0.4 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 N.D. +0.2 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.4 N.D. +0.1 +0.1
growth on ricinoleic acid YNB Glucose N.D. +0.4 N.D. +0.2 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.5 N.D. +0.4 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 N.D. +0.7 +0.1
auxotrophy YNB Glucose N.D. +0.6 N.D. +0.5 -0.0
auxotrophy Dropout Complete N.D. +0.6 N.D. +0.5 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. +0.4 N.D. +0.7 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +1.0 N.D. +0.5 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +1.0 N.D. +0.6 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +1.3 N.D. +0.3 +0.4
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