Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14084 and 14085 are separated by 312 nucleotides14085 and 14086 are separated by 146 nucleotides14086 and 14087 are separated by 277 nucleotides14087 and 14088 are separated by 384 nucleotides mRNA_5716: 14084 - Hypothetical Protein, at 759,003 to 760,646 14084 mRNA_5717: 14085 - K20524 LSB5 LAS seventeen-binding protein 5, at 760,959 to 763,265 14085 mRNA_5718: 14086 - SUPERFAMILY--SSF50370, at 763,412 to 765,448 14086 mRNA_5719: 14087 - K00290 LYS1 saccharopine dehydrogenase (NAD+, L-lysine forming), at 765,726 to 767,088 14087 mRNA_5720: 14088 - K04392 RAC1 Ras-related C3 botulinum toxin substrate 1, at 767,473 to 768,381 14088
Group Condition mRNA_5716 mRNA_5717 mRNA_5718 mRNA_5719 mRNA_5720
14084 14085 14086 14087 14088
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.5 -0.7 N.D. -1.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -1.3 +0.2 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.3 -0.1 -0.0 N.D. -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.4 -0.1 N.D. -1.3
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.1 -0.3 N.D. -0.1
growth on ypd YPD +0.1 -0.3 -0.6 N.D. +0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.3 -0.7 -0.9 N.D. +0.8
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.3 -0.6 N.D. +0.8
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.2 -0.6 N.D. +0.7
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.1 -0.5 N.D. +0.3
auxotrophy Dropout Complete +0.1 -0.5 -0.6 N.D. +1.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 -0.2 -0.2 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.3 +0.1 N.D. +0.3
auxotrophy YNB Glucose -0.0 -0.2 -0.5 N.D. +1.2
ypd buoyancy 40 hrs Low Buoyancy -0.3 -0.3 +0.1 N.D. +1.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.2 -0.2 N.D. +0.8
growth on oleic acid YNB Glucose +0.1 -0.2 -0.2 N.D. +1.1
growth on ricinoleic acid YNB Glucose -0.0 -0.2 +0.1 N.D. +1.5
growth on oleic acid YNB Oleic Acid +0.0 -0.1 -0.1 N.D. +2.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 +0.1 -0.1 N.D. +2.8
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.2 -0.1 N.D. +3.2
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