Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14085 and 14086 are separated by 146 nucleotides14086 and 14087 are separated by 277 nucleotides14087 and 14088 are separated by 384 nucleotides14088 and 14089 are separated by 350 nucleotides mRNA_5717: 14085 - K20524 LSB5 LAS seventeen-binding protein 5, at 760,959 to 763,265 14085 mRNA_5718: 14086 - SUPERFAMILY--SSF50370, at 763,412 to 765,448 14086 mRNA_5719: 14087 - K00290 LYS1 saccharopine dehydrogenase (NAD+, L-lysine forming), at 765,726 to 767,088 14087 mRNA_5720: 14088 - K04392 RAC1 Ras-related C3 botulinum toxin substrate 1, at 767,473 to 768,381 14088 mRNA_5721: 14089 - K19219 JMJD7 jumonji domain-containing protein 7, at 768,732 to 770,042 14089
Group Condition mRNA_5717 mRNA_5718 mRNA_5719 mRNA_5720 mRNA_5721
14085 14086 14087 14088 14089
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 -0.7 N.D. -1.0 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate -1.3 +0.2 N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 -0.1 N.D. -1.3 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.7 -0.9 N.D. +0.8 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.2 N.D. +0.1 -0.3
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.0 N.D. -0.4 +0.1
growth on ypd YPD -0.3 -0.6 N.D. +0.4 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.5 N.D. +0.3 -0.3
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.3 N.D. -0.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.3 +0.1 N.D. +0.3 +0.0
auxotrophy Dropout Complete -0.5 -0.6 N.D. +1.0 +0.2
auxotrophy YNB Glucose 75mM Arginine -0.3 -0.6 N.D. +0.8 +0.4
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.6 N.D. +0.7 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.2 N.D. +0.8 -0.2
auxotrophy YNB Glucose -0.2 -0.5 N.D. +1.2 +0.5
growth on oleic acid YNB Glucose -0.2 -0.2 N.D. +1.1 +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.3 +0.1 N.D. +1.2 +0.1
growth on ricinoleic acid YNB Glucose -0.2 +0.1 N.D. +1.5 +0.4
growth on oleic acid YNB Oleic Acid -0.1 -0.1 N.D. +2.0 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.1 N.D. +2.8 +0.5
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.1 N.D. +3.2 +0.6
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