Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14091 and 14092 are separated by 114 nucleotides14092 and 14093 are separated by 95 nucleotides14093 and 14094 are separated by 362 nucleotides14094 and 14095 are separated by 486 nucleotides mRNA_5723: 14091 - K15447 TRM44, METTL19 tRNASer (uridine44-2'-O)-methyltransferase, at 774,192 to 776,044 14091 mRNA_5724: 14092 - K05310 GPI7 ethanolaminephosphotransferase, at 776,159 to 779,515 14092 mRNA_5725: 14093 - K03234 EEF2 elongation factor 2, at 779,611 to 783,273 14093 mRNA_5726: 14094 - KOG2539 Mitochondrial/chloroplast ribosome small subunit component, at 783,636 to 785,944 14094 mRNA_5727: 14095 - HMMPfam-Elongation factor G C-terminus-PF00679,SUPERFAMILY--SSF54980, at 786,431 to 787,168 14095
Group Condition mRNA_5723 mRNA_5724 mRNA_5725 mRNA_5726 mRNA_5727
14091 14092 14093 14094 14095
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 -0.5 -1.4 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.2 -1.1 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 +0.2 -1.6 N.D. N.D.
growth on oleic acid YNB Glucose -0.1 -0.2 -0.6 N.D. N.D.
growth on oleic acid YNB Oleic Acid +0.7 -0.0 -1.5 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.9 -0.0 +0.1 N.D. N.D.
auxotrophy Dropout Complete -0.2 -0.4 -0.3 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.2 -0.3 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.1 -0.3 N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.6 +0.1 -1.0 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.0 -0.4 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy -0.3 +0.1 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 -0.4 +0.9 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.8 -0.2 -0.2 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.4 -0.2 +0.1 N.D. N.D.
auxotrophy YNB Glucose +0.5 -0.3 +0.5 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.8 +0.0 +0.3 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.2 +1.7 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 -0.2 +1.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.1 +1.5 N.D. N.D.
growth on ypd YPD +1.3 +0.0 +0.5 N.D. N.D.
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