Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14100 and 14101 overlap by 62 nucleotides14101 and 14102 are separated by 1653 nucleotides14102 and 14103 are separated by 464 nucleotides14103 and 14104 are separated by 564 nucleotides mRNA_5732: 14100 - HMMPfam-bZIP transcription factor-PF00170,HMMPfam-Minimal binding motif of Hap4 for binding to Hap2/3/5-PF10297,ProSitePatterns-Basic-leucine zipper (bZIP) domain signature.-PS00036,SUPERFAMILY--SSF57959, at 798,563 to 800,717 14100 mRNA_5733: 14101 - KOG4197 FOG PPR repeat, at 800,656 to 803,521 14101 mRNA_5734: 14102 - K01183 E3.2.1.14 chitinase, at 805,175 to 807,034 14102 mRNA_5735: 14103 - K12828 SF3B1, SAP155 splicing factor 3B subunit 1, at 807,499 to 811,509 14103 mRNA_5736: 14104 - K03250 EIF3E, INT6 translation initiation factor 3 subunit E, at 812,074 to 813,868 14104
Group Condition mRNA_5732 mRNA_5733 mRNA_5734 mRNA_5735 mRNA_5736
14100 14101 14102 14103 14104
buoyancy on lipid accumulation 40 hrs Median Buoyancy -2.7 -0.1 +0.7 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.5 -0.5 -0.5 N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.6 -0.1 -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -1.0 -0.0 +0.4 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.5 -0.2 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -1.0 -0.3 +0.7 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.0 -0.2 -0.3 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 +0.5 -0.6 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 +0.3 -0.3 N.D. N.D.
growth on ricinoleic acid YNB Glucose -0.8 +0.7 +0.1 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -1.4 +0.3 +1.0 N.D. N.D.
growth on oleic acid YNB Glucose -0.1 +0.1 +0.1 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +1.2 -0.3 -0.8 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.2 +0.5 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.3 -0.3 +1.0 N.D. N.D.
growth on ypd YPD +0.9 -0.1 -0.5 N.D. N.D.
auxotrophy Dropout Complete -1.0 +0.2 +1.2 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.7 +0.1 +1.1 N.D. N.D.
auxotrophy YNB Glucose -1.0 +0.3 +1.4 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.1 +0.7 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -0.4 +0.2 +1.4 N.D. N.D.
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