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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
14100 and 14101 overlap by 62 nucleotides
14101 and 14102 are separated by 1653 nucleotides
14102 and 14103 are separated by 464 nucleotides
14103 and 14104 are separated by 564 nucleotides
mRNA_5732: 14100 - HMMPfam-bZIP transcription factor-PF00170,HMMPfam-Minimal binding motif of Hap4 for binding to Hap2/3/5-PF10297,ProSitePatterns-Basic-leucine zipper (bZIP) domain signature.-PS00036,SUPERFAMILY--SSF57959, at 798,563 to 800,717
14100
mRNA_5733: 14101 - KOG4197 FOG PPR repeat, at 800,656 to 803,521
14101
mRNA_5734: 14102 - K01183 E3.2.1.14 chitinase, at 805,175 to 807,034
14102
mRNA_5735: 14103 - K12828 SF3B1, SAP155 splicing factor 3B subunit 1, at 807,499 to 811,509
14103
mRNA_5736: 14104 - K03250 EIF3E, INT6 translation initiation factor 3 subunit E, at 812,074 to 813,868
14104
Group
Condition
mRNA
_5732
mRNA
_5733
mRNA
_5734
mRNA
_5735
mRNA
_5736
14100
14101
14102
14103
14104
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-2.7
-0.1
+0.7
N.D.
N.D.
facs on lipid accumulation
Sorted No BODIPY Gate
-0.5
-0.5
-0.5
N.D.
N.D.
growth on oleic acid
YNB Oleic Acid
-0.6
-0.1
-0.1
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs High Buoyancy
-1.0
-0.0
+0.4
N.D.
N.D.
facs on lipid accumulation
Sorted High BODIPY Gate
-0.5
-0.2
+0.1
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-1.0
-0.3
+0.7
N.D.
N.D.
ypd buoyancy
40 hrs High Buoyancy
+0.0
-0.2
-0.3
N.D.
N.D.
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.3
+0.5
-0.6
N.D.
N.D.
ypd buoyancy
40 hrs No Buoyancy Separation
-0.3
+0.3
-0.3
N.D.
N.D.
growth on ricinoleic acid
YNB Glucose
-0.8
+0.7
+0.1
N.D.
N.D.
growth on ricinoleic acid
YNB Methylricinoleic Acid
-1.4
+0.3
+1.0
N.D.
N.D.
growth on oleic acid
YNB Glucose
-0.1
+0.1
+0.1
N.D.
N.D.
facs on lipid accumulation
Sorted Low BODIPY Gate
+1.2
-0.3
-0.8
N.D.
N.D.
ypd buoyancy
40 hrs Median Buoyancy
-0.1
-0.2
+0.5
N.D.
N.D.
ypd buoyancy
40 hrs Low Buoyancy
-0.3
-0.3
+1.0
N.D.
N.D.
growth on ypd
YPD
+0.9
-0.1
-0.5
N.D.
N.D.
auxotrophy
Dropout Complete
-1.0
+0.2
+1.2
N.D.
N.D.
auxotrophy
YNB Glucose 75mM Arginine
-0.7
+0.1
+1.1
N.D.
N.D.
auxotrophy
YNB Glucose
-1.0
+0.3
+1.4
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.1
-0.1
+0.7
N.D.
N.D.
auxotrophy
YNB Glucose 75mM Methionine
-0.4
+0.2
+1.4
N.D.
N.D.
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