Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14105 and 14106 are separated by 326 nucleotides14106 and 14107 are separated by 816 nucleotides14107 and 14108 are separated by 448 nucleotides14108 and 14109 are separated by 1203 nucleotides mRNA_5737: 14105 - HMMPfam-Arsenite-resistance protein 2-PF04959,ProSitePatterns-Zinc finger C2H2 type domain signature.-PS00028,ProSiteProfiles-Zinc finger C2H2 type domain profile.-PS50157, at 814,354 to 815,055 14105 mRNA_5738: 14106 - SUPERFAMILY--SSF54928, at 815,382 to 817,330 14106 mRNA_5739: 14107 - K05692 ACTB_G1 actin beta/gamma 1, at 818,147 to 820,488 14107 mRNA_5740: 14108 - K00121 frmA, ADH5, adhC S-(hydroxymethyl)glutathione dehydrogenase / alcohol dehydrogenase, at 820,937 to 822,719 14108 mRNA_5741: 14109 - K00121 frmA, ADH5, adhC S-(hydroxymethyl)glutathione dehydrogenase / alcohol dehydrogenase, at 823,923 to 825,708 14109
Group Condition mRNA_5737 mRNA_5738 mRNA_5739 mRNA_5740 mRNA_5741
14105 14106 14107 14108 14109
growth on oleic acid YNB Oleic Acid -1.7 -1.4 N.D. +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -1.6 -1.5 N.D. +0.3 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -2.1 -0.8 N.D. +0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -1.1 -1.7 N.D. +0.2 N.D.
auxotrophy Dropout Complete -0.9 -1.4 N.D. -0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.7 -1.3 N.D. -0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.8 -0.9 N.D. -0.4 N.D.
auxotrophy YNB Glucose -0.8 -0.8 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -1.0 -1.0 N.D. +0.3 N.D.
growth on oleic acid YNB Glucose -0.6 -0.9 N.D. -0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -1.1 -0.2 N.D. +0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.8 -0.6 N.D. +0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 +0.2 N.D. -0.7 N.D.
growth on ricinoleic acid YNB Glucose -0.4 -0.5 N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 +0.0 N.D. -0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.4 +0.2 N.D. -0.0 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.1 N.D. +0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.7 +0.2 N.D. +0.3 N.D.
growth on ypd YPD +2.1 -1.0 N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.8 +0.5 N.D. +0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +1.0 +1.2 N.D. -0.3 N.D.
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