Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14106 and 14107 are separated by 816 nucleotides14107 and 14108 are separated by 448 nucleotides14108 and 14109 are separated by 1203 nucleotides14109 and 14110 are separated by 1784 nucleotides mRNA_5738: 14106 - SUPERFAMILY--SSF54928, at 815,382 to 817,330 14106 mRNA_5739: 14107 - K05692 ACTB_G1 actin beta/gamma 1, at 818,147 to 820,488 14107 mRNA_5740: 14108 - K00121 frmA, ADH5, adhC S-(hydroxymethyl)glutathione dehydrogenase / alcohol dehydrogenase, at 820,937 to 822,719 14108 mRNA_5741: 14109 - K00121 frmA, ADH5, adhC S-(hydroxymethyl)glutathione dehydrogenase / alcohol dehydrogenase, at 823,923 to 825,708 14109 mRNA_5742: 14110 - BLAST spindle pole body protein [Moesziomyces antarcticus T..., at 827,493 to 828,317 14110
Group Condition mRNA_5738 mRNA_5739 mRNA_5740 mRNA_5741 mRNA_5742
14106 14107 14108 14109 14110
auxotrophy Dropout Complete -1.4 N.D. -0.1 N.D. -0.8
auxotrophy YNB Glucose 75mM Methionine -1.3 N.D. -0.2 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine -0.9 N.D. -0.4 N.D. -0.3
auxotrophy YNB Glucose -0.8 N.D. -0.2 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -1.7 N.D. +0.2 N.D. +0.3
growth on oleic acid YNB Oleic Acid -1.4 N.D. +0.2 N.D. +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -1.5 N.D. +0.3 N.D. +0.5
growth on ypd YPD -1.0 N.D. +0.2 N.D. +0.1
growth on oleic acid YNB Glucose -0.9 N.D. -0.1 N.D. +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -1.0 N.D. +0.3 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.8 N.D. +0.2 N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. -0.0 N.D. -0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. +0.2 N.D. -0.2
growth on ricinoleic acid YNB Glucose -0.5 N.D. +0.3 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.2 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +1.2 N.D. -0.3 N.D. -0.6
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. +0.3 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. -0.7 N.D. +0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. -0.0 N.D. +0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.6 N.D. +0.4 N.D. +0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. +0.3 N.D. -0.1
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