Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14110 and 14111 are separated by 224 nucleotides14111 and 14112 are separated by 720 nucleotides14112 and 14113 are separated by 822 nucleotides14113 and 14114 are separated by 1299 nucleotides mRNA_5742: 14110 - BLAST spindle pole body protein [Moesziomyces antarcticus T..., at 827,493 to 828,317 14110 mRNA_5743: 14111 - K05672 ABCC12 ATP-binding cassette, subfamily C (CFTR/MRP), member 12, at 828,542 to 833,992 14111 mRNA_5744: 14112 - KOG0250 DNA repair protein RAD18 (SMC family protein), at 834,713 to 839,482 14112 mRNA_5745: 14113 - K11665 INO80, INOC1 DNA helicase INO80, at 840,305 to 845,790 14113 mRNA_5746: 14114 - Hypothetical Protein, at 847,090 to 848,966 14114
Group Condition mRNA_5742 mRNA_5743 mRNA_5744 mRNA_5745 mRNA_5746
14110 14111 14112 14113 14114
facs on lipid accumulation Sorted No BODIPY Gate +0.7 +0.1 N.D. -1.8 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 +0.1 N.D. -1.4 -0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.2 N.D. -1.0 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.0 N.D. -0.5 -0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.1 N.D. -1.4 -0.2
growth on ypd YPD +0.1 +0.1 N.D. -0.3 -0.6
growth on oleic acid YNB Glucose +0.3 +0.1 N.D. -0.5 -0.4
growth on ricinoleic acid YNB Glucose +0.2 -0.0 N.D. -0.7 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.3 +0.1 N.D. -0.4 +0.4
auxotrophy Dropout Complete -0.8 +0.2 N.D. +0.1 +0.3
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.2 N.D. -0.2 -0.0
auxotrophy YNB Glucose -0.2 +0.1 N.D. -0.1 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -0.3 N.D. +0.0 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 +0.2 N.D. +0.1 +0.5
facs on lipid accumulation Sorted High BODIPY Gate +0.3 +0.1 N.D. +0.2 -0.4
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.0 N.D. +0.4 +0.0
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.0 N.D. +0.6 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.5 -0.1 N.D. +1.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 +0.0 N.D. +0.1 +0.2
growth on oleic acid YNB Oleic Acid +0.4 +0.1 N.D. +0.3 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.4 N.D. +0.6 +0.4
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