Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14135 and 14136 are separated by 833 nucleotides14136 and 14137 are separated by 2914 nucleotides14137 and 14138 are separated by 476 nucleotides14138 and 14139 are separated by 250 nucleotides mRNA_5767: 14135 - K02606 ORC4 origin recognition complex subunit 4, at 890,785 to 894,210 14135 mRNA_5768: 14136 - Hypothetical Protein, at 895,044 to 899,242 14136 mRNA_5769: 14137 - Hypothetical Protein, at 902,157 to 903,800 14137 mRNA_5770: 14138 - K15171 SUPT4H1, SPT4 transcription elongation factor SPT4, at 904,277 to 904,949 14138 mRNA_5771: 14139 - K00921 PIKFYVE, FAB1 1-phosphatidylinositol-3-phosphate 5-kinase, at 905,200 to 913,412 14139
Group Condition mRNA_5767 mRNA_5768 mRNA_5769 mRNA_5770 mRNA_5771
14135 14136 14137 14138 14139
growth on ypd YPD N.D. +0.1 -0.6 -0.3 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 +0.7 -1.6 -0.4
auxotrophy Dropout Complete N.D. +0.2 -0.6 -0.7 +0.1
auxotrophy YNB Glucose N.D. +0.2 -0.7 +0.1 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 +0.1 -0.0 -0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 +0.5 -0.4 -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 -0.3 +0.3 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.0 -0.3 +0.8 -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.3 +0.3 -0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 +0.1 +0.4 +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 -0.2 +0.1 +0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 +0.3 +0.5 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 +0.1 +0.5 -0.3
growth on oleic acid YNB Glucose N.D. +0.1 +0.1 +0.3 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.0 +0.1 +0.5 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 +0.4 +0.0 +0.0
growth on oleic acid YNB Oleic Acid N.D. +0.3 +0.3 +0.0 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 +0.1 +0.8 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 +0.4 +0.4 +0.3
growth on ricinoleic acid YNB Glucose N.D. +0.3 +0.3 +0.7 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 +0.5 +1.3 -0.1
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