Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14142 and 14143 are separated by 292 nucleotides14143 and 14144 are separated by 360 nucleotides14144 and 14145 are separated by 185 nucleotides14145 and 14146 are separated by 566 nucleotides mRNA_5774: 14142 - K11982 RNF115_126 E3 ubiquitin-protein ligase RNF115/126, at 918,001 to 918,584 14142 mRNA_5775: 14143 - Hypothetical Protein, at 918,877 to 919,776 14143 mRNA_5776: 14144 - HMMPfam-Phosphatidylinositol-specific phospholipase C, X domain-PF00388,ProSiteProfiles-Phosphatidylinositol-specific phospholipase X-box domain profile.-PS50007,SUPERFAMILY--SSF51695, at 920,137 to 921,765 14144 mRNA_5777: 14145 - Hypothetical Protein, at 921,951 to 922,623 14145 mRNA_5778: 14146 - HMMPfam-bZIP transcription factor-PF00170,HMMPfam-Transcription factor PAP1-PF08601,ProSitePatterns-Basic-leucine zipper (bZIP) domain signature.-PS00036,ProSiteProfiles-Basic-leucine zipper (bZIP) domain profile.-PS50217,SMART-basic region leucin zipper-SM00338,SUPERFAMILY--SSF111430,SUPERFAMILY--SSF57959, at 923,190 to 924,639 14146
Group Condition mRNA_5774 mRNA_5775 mRNA_5776 mRNA_5777 mRNA_5778
14142 14143 14144 14145 14146
ypd buoyancy 40 hrs No Buoyancy Separation +0.9 -0.5 -0.1 +0.3 -1.5
facs on lipid accumulation Sorted High BODIPY Gate -0.5 +0.2 -0.1 +0.1 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.6 +0.0 +0.5 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.5 -0.3 +0.0 -0.3 -0.2
growth on ypd YPD +0.3 -0.1 +0.2 -0.2 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 +0.4 -0.7 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.1 +0.1 +0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.5 +0.0 +0.2 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +0.2 +0.1 +0.2 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.5 -0.2 +0.2 +0.3 +0.7
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.4 +0.2 -0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.2 -0.0 +0.3 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.8 +0.7 -0.5 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.1 +0.5 +0.3 +0.2
growth on oleic acid YNB Oleic Acid +0.4 +0.2 +0.0 +0.2 +0.3
auxotrophy YNB Glucose 75mM Methionine +0.0 +0.4 -0.0 +0.6 +0.3
auxotrophy YNB Glucose -0.1 +0.3 +0.1 +0.5 +0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.5 -0.1 +0.5 +0.4
growth on ricinoleic acid YNB Glucose +0.3 +0.5 +0.2 +0.3 +0.3
growth on oleic acid YNB Glucose +0.1 +0.4 +0.4 +0.3 +0.4
auxotrophy Dropout Complete +0.8 +0.1 -0.1 +0.4 +0.8
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