Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14157 and 14158 are separated by 96 nucleotides14158 and 14159 are separated by 552 nucleotides14159 and 14160 are separated by 256 nucleotides14160 and 14161 are separated by 174 nucleotides mRNA_5789: 14157 - KOG2859 DNA repair protein, member of the recA/RAD51 family, at 944,648 to 945,829 14157 mRNA_5790: 14158 - K01054 MGLL acylglycerol lipase, at 945,926 to 947,446 14158 mRNA_5791: 14159 - K15115 SLC25A32, MFT solute carrier family 25 (mitochondrial folate transporter), member 32, at 947,999 to 949,301 14159 mRNA_5792: 14160 - K10755 RFC2_4 replication factor C subunit 2/4, at 949,558 to 951,302 14160 mRNA_5793: 14161 - K10581 UBE2O ubiquitin-conjugating enzyme E2 O, at 951,477 to 955,108 14161
Group Condition mRNA_5789 mRNA_5790 mRNA_5791 mRNA_5792 mRNA_5793
14157 14158 14159 14160 14161
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.1 N.D. N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.2 N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.0 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.0 N.D. N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 -0.1 N.D. N.D. +0.0
growth on oleic acid YNB Glucose -0.1 -0.1 N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 -0.1 N.D. N.D. +0.1
growth on ricinoleic acid YNB Glucose -0.1 -0.0 N.D. N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.1 N.D. N.D. +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.1 N.D. N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 +0.2 N.D. N.D. +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.0 N.D. N.D. +0.0
growth on oleic acid YNB Oleic Acid -0.2 +0.1 N.D. N.D. +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.0 N.D. N.D. +0.1
growth on ypd YPD -0.0 +0.2 N.D. N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +0.0 N.D. N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 +0.3 N.D. N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine +0.4 -0.1 N.D. N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine +0.5 +0.0 N.D. N.D. +0.1
auxotrophy Dropout Complete +0.4 -0.0 N.D. N.D. +0.2
auxotrophy YNB Glucose +0.6 +0.0 N.D. N.D. +0.1
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