Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14158 and 14159 are separated by 552 nucleotides14159 and 14160 are separated by 256 nucleotides14160 and 14161 are separated by 174 nucleotides14161 and 14162 are separated by 364 nucleotides mRNA_5790: 14158 - K01054 MGLL acylglycerol lipase, at 945,926 to 947,446 14158 mRNA_5791: 14159 - K15115 SLC25A32, MFT solute carrier family 25 (mitochondrial folate transporter), member 32, at 947,999 to 949,301 14159 mRNA_5792: 14160 - K10755 RFC2_4 replication factor C subunit 2/4, at 949,558 to 951,302 14160 mRNA_5793: 14161 - K10581 UBE2O ubiquitin-conjugating enzyme E2 O, at 951,477 to 955,108 14161 mRNA_5794: 14162 - K01637 E4.1.3.1, aceA isocitrate lyase, at 955,473 to 957,559 14162
Group Condition mRNA_5790 mRNA_5791 mRNA_5792 mRNA_5793 mRNA_5794
14158 14159 14160 14161 14162
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. N.D. +0.2 -1.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. N.D. -0.1 -0.7
facs on lipid accumulation Sorted High BODIPY Gate +0.0 N.D. N.D. +0.1 -1.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. N.D. +0.0 -0.6
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. N.D. +0.0 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. N.D. +0.1 -0.4
growth on oleic acid YNB Oleic Acid +0.1 N.D. N.D. +0.3 -0.6
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. N.D. +0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.0 N.D. N.D. +0.0 -0.1
growth on oleic acid YNB Glucose -0.1 N.D. N.D. +0.2 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. N.D. +0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. N.D. +0.1 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. N.D. +0.2 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 N.D. N.D. +0.1 +0.2
growth on ypd YPD +0.2 N.D. N.D. +0.1 -0.1
growth on ricinoleic acid YNB Glucose -0.0 N.D. N.D. +0.2 +0.1
auxotrophy YNB Glucose +0.0 N.D. N.D. +0.1 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. N.D. +0.1 +0.3
auxotrophy Dropout Complete -0.0 N.D. N.D. +0.2 +0.3
auxotrophy YNB Glucose 75mM Arginine +0.0 N.D. N.D. +0.1 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. N.D. +0.2 +0.9
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