Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14160 and 14161 are separated by 174 nucleotides14161 and 14162 are separated by 364 nucleotides14162 and 14163 are separated by 295 nucleotides14163 and 14164 overlap by 20 nucleotides mRNA_5792: 14160 - K10755 RFC2_4 replication factor C subunit 2/4, at 949,558 to 951,302 14160 mRNA_5793: 14161 - K10581 UBE2O ubiquitin-conjugating enzyme E2 O, at 951,477 to 955,108 14161 mRNA_5794: 14162 - K01637 E4.1.3.1, aceA isocitrate lyase, at 955,473 to 957,559 14162 mRNA_5795: 14163 - KOG4842 Protein involved in sister chromatid separation and/or segregation, at 957,855 to 959,394 14163 mRNA_5796: 14164 - BLAST Protein of unknown function DUF4598 [Xanthophyllomyce..., at 959,375 to 960,569 14164
Group Condition mRNA_5792 mRNA_5793 mRNA_5794 mRNA_5795 mRNA_5796
14160 14161 14162 14163 14164
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 -0.7 -0.4 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 -1.0 -0.4 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 -0.4 -0.3 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 -1.1 -0.0 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 -0.4 -0.2 +0.0
growth on oleic acid YNB Oleic Acid N.D. +0.3 -0.6 -0.0 -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.0 -0.1 +0.2 -0.4
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 -0.3 +0.1 -0.1
growth on oleic acid YNB Glucose N.D. +0.2 -0.1 -0.2 -0.1
auxotrophy Dropout Complete N.D. +0.2 +0.3 -0.5 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 +0.2 -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 -0.0 -0.0 -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 -0.1 -0.3 +0.4
auxotrophy YNB Glucose N.D. +0.1 +0.3 -0.5 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 +0.3 -0.3 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.0 -0.6 +0.5 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 +0.1 -0.0 +0.2
growth on ypd YPD N.D. +0.1 -0.1 +0.2 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 +0.4 -0.2 +0.3
growth on ricinoleic acid YNB Glucose N.D. +0.2 +0.1 +0.1 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 +0.9 +0.0 -0.4
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