Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14165 and 14166 are separated by 256 nucleotides14166 and 14167 are separated by 216 nucleotides14167 and 14168 are separated by 334 nucleotides14168 and 14169 are separated by 265 nucleotides mRNA_5797: 14165 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 962,183 to 965,371 14165 mRNA_5798: 14166 - KOG3433 Protein involved in meiotic recombination/predicted coiled-coil protein, at 965,628 to 966,973 14166 mRNA_5799: 14167 - K09540 SEC63, DNAJC23 translocation protein SEC63, at 967,190 to 969,688 14167 mRNA_5800: 14168 - K11801 WDR23 WD repeat-containing protein 23, at 970,023 to 972,593 14168 mRNA_5801: 14169 - HMMPfam-Helix-loop-helix DNA-binding domain-PF00010,ProSiteProfiles-Myc-type, basic helix-loop-helix (bHLH) domain profile.-PS50888,SMART-helix loop helix domain-SM00353,SUPERFAMILY--SSF47459, at 972,859 to 974,663 14169
Group Condition mRNA_5797 mRNA_5798 mRNA_5799 mRNA_5800 mRNA_5801
14165 14166 14167 14168 14169
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 -0.1 N.D. -0.2 -1.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.2 N.D. -0.2 -0.1
growth on ypd YPD -0.4 -0.0 N.D. -0.0 -0.1
growth on oleic acid YNB Oleic Acid -0.4 +0.2 N.D. +0.2 -0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.0 N.D. -0.2 +0.0
auxotrophy YNB Glucose -0.1 +0.1 N.D. -0.2 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 -0.1 N.D. +0.3 -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.1 N.D. +0.1 +0.0
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.2 N.D. +0.0 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.8 -0.2 N.D. -0.2 -0.0
growth on oleic acid YNB Glucose +0.2 -0.1 N.D. +0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.3 N.D. +0.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.1 N.D. +0.0 +0.4
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.6 N.D. +0.1 -0.1
auxotrophy Dropout Complete -0.1 +0.5 N.D. +0.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 +0.4 N.D. +0.2 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.9 -0.0 N.D. -0.2 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 +0.3 N.D. -0.2 +0.1
growth on ricinoleic acid YNB Glucose +0.3 +0.4 N.D. -0.0 +0.0
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.3 N.D. +0.2 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 +0.5 N.D. -0.1 -0.0
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