Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14167 and 14168 are separated by 334 nucleotides14168 and 14169 are separated by 265 nucleotides14169 and 14170 are separated by 156 nucleotides14170 and 14171 are separated by 326 nucleotides mRNA_5799: 14167 - K09540 SEC63, DNAJC23 translocation protein SEC63, at 967,190 to 969,688 14167 mRNA_5800: 14168 - K11801 WDR23 WD repeat-containing protein 23, at 970,023 to 972,593 14168 mRNA_5801: 14169 - HMMPfam-Helix-loop-helix DNA-binding domain-PF00010,ProSiteProfiles-Myc-type, basic helix-loop-helix (bHLH) domain profile.-PS50888,SMART-helix loop helix domain-SM00353,SUPERFAMILY--SSF47459, at 972,859 to 974,663 14169 mRNA_5802: 14170 - K07441 ALG14 beta-1,4-N-acetylglucosaminyltransferase, at 974,820 to 975,968 14170 mRNA_5803: 14171 - Hypothetical Protein, at 976,295 to 977,367 14171
Group Condition mRNA_5799 mRNA_5800 mRNA_5801 mRNA_5802 mRNA_5803
14167 14168 14169 14170 14171
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 -1.0 -0.6 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 -0.4 -0.7 -0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 +0.0 -0.5 -0.3
growth on oleic acid YNB Oleic Acid N.D. +0.2 -0.4 -0.4 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 -0.0 +0.0 -0.3
growth on ypd YPD N.D. -0.0 -0.1 -0.2 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 +0.2 -0.6 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 -0.0 +0.0 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 -0.1 -0.1 +0.1
auxotrophy YNB Glucose N.D. -0.2 +0.1 -0.3 +0.1
auxotrophy Dropout Complete N.D. +0.1 +0.1 -0.1 -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 +0.2 -0.2 -0.2
growth on oleic acid YNB Glucose N.D. +0.1 +0.2 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.3 -0.0 -0.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 -0.0 +0.2 -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 -0.1 -0.2 +0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.0 +0.4 -0.2 -0.0
growth on ricinoleic acid YNB Glucose N.D. -0.0 +0.0 -0.1 +0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 +0.0 -0.1 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.2 +0.1 +0.4 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 +0.0 +0.1 +0.5
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