Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14179 and 14180 are separated by 255 nucleotides14180 and 14181 are separated by 189 nucleotides14181 and 14182 are separated by 168 nucleotides14182 and 14183 are separated by 380 nucleotides mRNA_5811: 14179 - K01599 hemE, UROD uroporphyrinogen decarboxylase, at 992,194 to 993,567 14179 mRNA_5812: 14180 - K02831 RAD53 ser/thr/tyr protein kinase RAD53, at 993,823 to 996,800 14180 mRNA_5813: 14181 - K15340 DCLRE1A, SNM1A, PSO2 DNA cross-link repair 1A protein, at 996,990 to 1,000,538 14181 mRNA_5814: 14182 - K06669 SMC3, CSPG6 structural maintenance of chromosome 3 (chondroitin sulfate proteoglycan 6), at 1,000,707 to 1,005,630 14182 mRNA_5815: 14183 - KOG2614 Kynurenine 3-monooxygenase and related flavoprotein monooxygenases, at 1,006,011 to 1,008,191 14183
Group Condition mRNA_5811 mRNA_5812 mRNA_5813 mRNA_5814 mRNA_5815
14179 14180 14181 14182 14183
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.3 +0.0 N.D. -0.8
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 -0.1 N.D. -0.7
growth on oleic acid YNB Oleic Acid N.D. -0.1 -0.1 N.D. -0.7
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 -0.2 N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 -0.4 N.D. +0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 -0.2 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.4 +0.0 N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. -0.4 -0.1 N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.3 -0.3 N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. -0.5 -0.1 N.D. +0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.0 -0.4 N.D. +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.4 -0.1 N.D. +0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 -0.0 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 +0.0 N.D. +0.1
auxotrophy YNB Glucose N.D. -0.3 +0.1 N.D. +0.3
growth on ypd YPD N.D. -0.1 +0.4 N.D. -0.0
auxotrophy Dropout Complete N.D. -0.4 +0.2 N.D. +0.5
growth on ricinoleic acid YNB Glucose N.D. -0.2 +0.2 N.D. +0.2
growth on oleic acid YNB Glucose N.D. +0.0 +0.1 N.D. +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 +0.1 N.D. +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 +0.2 N.D. +0.8
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