Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 14182 and 14183 are separated by 380 nucleotides14183 and 14184 are separated by 1450 nucleotides14184 and 14185 are separated by 173 nucleotides14185 and 14186 are separated by 636 nucleotides mRNA_5814: 14182 - K06669 SMC3, CSPG6 structural maintenance of chromosome 3 (chondroitin sulfate proteoglycan 6), at 1,000,707 to 1,005,630 14182 mRNA_5815: 14183 - KOG2614 Kynurenine 3-monooxygenase and related flavoprotein monooxygenases, at 1,006,011 to 1,008,191 14183 mRNA_5816: 14184 - KOG1437 Fasciclin and related adhesion glycoproteins, at 1,009,642 to 1,012,931 14184 mRNA_5817: 14185 - K09680 coaW type II pantothenate kinase, at 1,013,105 to 1,015,263 14185 mRNA_5818: 14186 - K14857 SPB1, FTSJ3 AdoMet-dependent rRNA methyltransferase SPB1, at 1,015,900 to 1,019,364 14186
Group Condition mRNA_5814 mRNA_5815 mRNA_5816 mRNA_5817 mRNA_5818
14182 14183 14184 14185 14186
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.7 -0.2 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.8 -0.1 N.D. N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.7 +0.0 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.3 -0.0 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 +0.2 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 -0.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.0 +0.1 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 +0.2 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 +0.1 N.D. N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.2 -0.1 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 -0.1 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 +0.0 N.D. N.D.
growth on ypd YPD N.D. -0.0 +0.2 N.D. N.D.
growth on oleic acid YNB Glucose N.D. +0.4 -0.1 N.D. N.D.
auxotrophy YNB Glucose N.D. +0.3 +0.1 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. +0.3 +0.1 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.3 +0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.4 +0.2 N.D. N.D.
auxotrophy Dropout Complete N.D. +0.5 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.5 +0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.8 +0.2 N.D. N.D.
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